We developed a one-step real-time duplex reverse transcription PCR (RT-PCR) method using the LightCycler platform. This method allows simultaneous reverse transcription and real-time PCR amplification of two mRNAs of specific genes of interest (analyte genes) and mRNA of constantly transcribed genes (housekeeping genes) in a single-tube reaction. Specimen total nucleic acids were used because e...

Housekeeping genes (HKGs) generally have fundamental functions in basic biochemical processes in organisms, and usually have relatively steady expression levels across various tissues. They play an important role in the normalization of microarray technology. Using Fourier analysis we transformed gene expression time-series from a Hela cell cycle gene expression dataset into Fourier spectra, an...

Using a collection of expressed sequence tag (EST) data, we re-evaluated the correlation of tissue specificity with genomic structure, phyletic age, evolutionary rate and promoter architecture of human genes. We found that housekeeping genes are less compact and older than tissue-specific genes, and they evolve more slowly in terms of both coding and core promoter sequences. Housekeeping genes ...

Mammalian gene expression is usually carried out at the level of mRNA where the amount of mRNA of interest is measured under different conditions such as growth and development. It is therefore important to use a "housekeeping gene", that does not change in relative abundance during the experimental conditions, as a standard or internal control. However, recent data suggest that expression of s...

BACKGROUND We previously reported that specialized and housekeeping genes are differentially regulated via de novo recruitment and pause-release of RNA polymerase II, respectively, during cardiac hypertrophy. However, the significance of this finding remains to be examined. Therefore, the purpose of this study was to determine the mechanisms that differentially regulate these gene groups and ex...

background & aim: job conflict can impact different aspects of work and quality of the presented services. the present study was carried out to determine the level of job conflict and its management styles among the employed nurses. methods & materials : this descriptive cross-sectional study was carried out on 423 employed nurses in different units of the educational hospitals of kurdistan uni...

view. in this paper, the question of influence in the south caucasus and europe :union: foreign policy . a. iran 's regional interests can be defined both in terms of cooperation or conflict , has developed the hypothesis that the objectives and activities of the :union: of europe in the caucasus show that not only the conflict of the conflict between the interests of and the there is no :...