BACKGROUND The objectives of this study were to assess the heterogeneities of estimates and to estimate the seroprevalence of brucellosis in animals and humans in Ethiopia. METHODS/PRINCIPAL FINDINGS Data from 70 studies covering 75879 animals and 2223 humans were extracted. Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT) in series were the most frequently used serological te...

The complement fixation test is of great diagnostic and prognostic value in the study of suspected cases of blastomycosis, coccidioidomycosis, and histoplasmosis. As the number of requests for these tests continues to grow along with the increasing awareness of the public health importance of these diseases, the need to produce large stocks of antigens more efficiently has arisen. Accordingly, ...

WORTHINGTON, R. W., MULDERS, MARIA S. G., McFARLANE I. S. and BECKER DAPHNE. Serological titres following vaccination of sheep and goats with B. :nelitensis Rev 1 vaccine: Onderstepoort f. vet. Res. 40(1), 1-6 (1973) Rev 1 vaccination of sheep induced complement fixation titres to both Brucella abortus and Brucella ovis antigens, the complement fixing antibody titres were, however, higher with ...

The aim of the study was an evaluation of fluorescence polarisation assay (FPA) as a potential tool improving specificity of serological diagnosis of brucellosis in pigs. The evaluation was done by comparing the results of FPA with the results of rose bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and ELISA when false positive sera were tested. One hundred nin...

A Case of Cryptococcal meningitis was diagnosed in a 22 year old Pigeon -fancier from Mazandaran prm ince of Iran (close to the Caspian sea). The patient was treated with systemic 5-nuoc to,;in and amphotericin B together with intrathecal injection of ampho. B. Further investigations showed an abnormal reduction in the number ofT-lymphocytes, while humoral immunity was maintained in the normal ...

The screening Rose Bengal test (RBT), the buffered plate agglutination test (BPAT), and the confirmatory complement fixation test (CFT) are currently approved by the World Organization for Animal Health (OIE) for diagnosis of goat brucellosis. However, RBT (at 3% or 8% cell concentration) is known to be affected by vaccinal antibodies. In the present study, Mexican and Canadian OIE tests were c...

A competitive enzyme immunoassay was developed to detect serum complement-fixing antibodies in virus diseases. This assay utilized conglutinin-covered plastic beads as the solid phase to detect specific antibody-antigen complexes that competed for complement with a probe complex comprised of Escherichia coli beta-galactosidase and its specific antibody. Binding to the solid phase is C3bi mediat...

It is shown by complement fixation studies with protein antigens and specific immune rabbit sera that the rate of fixation of complement is determined by the concentration of antibodies in the immune sera, that the greater part of fixation of complement takes place during the 1st hour, and that fixation is practically completed at the end of 4 hours at ice box temperature. It is further shown t...

Heart immunofluorescent autoantibody tests, the complement fixation test for Chagas' disease, the hemagglutination test for toxoplasmosis, and the latex flocculation test for rheumatoid disease were compared in 34 patients with primary myocardial disease and their paired control subjects. There was no difference in the incidence of elevated titers or in the actual titer levels between the group...