Immunoglobulin synthesis in response to experimental infection of cattle with Theileria parva (Muguga) was studied by Sephadex G 200 chromatography. Serum samples were collected for 88 days following inoculation. Indirect fluorescent antibody (IFA), complement fixation (CF), indirect haemagglutination (IHA) and immunodiffusion (ID) tests were used to detect activity against T. parva piroplasm a...

Antibody-dependent cellular cytotoxicity (ADCC) and complement fixation both appear to play a role in mediating antitumor effects of monoclonal antibodies (mAbs), including rituximab. We evaluated the relationship between rituximab-induced complement fixation, natural killer (NK)-cell activation, and NK cell-mediated ADCC. Down-modulation of NK- cell CD16 and NK-cell activation induced by ritux...

Chemical fixation is nearly indispensable in the biological sciences, especially in circumstances where cryo-fixation is not applicable. While universally employed for the preservation of cell organization, chemical fixatives often introduce artifacts that can confound identification of true structures. Since biological research is increasingly probing ever-finer details of the cellular archite...

It was confirmed that the immune adherence hemagglutination (IAHA) test might be useful for the measurement of antigens and antibodies of some animal viruses. A suitable procedure of IAHA for the titration of animal virus and corresponding antibody was determined. No positive reaction of IAHA could be seen with any kind of primate erythrocytes except for human erythrocytes, and the guinea pig c...

Since the publication of the original complementfixation test for syphilis by Wassermann, Neisser, Bruck, and Schucht (1906) and the publication of the flocculation test of Porges and Meier (1908), innumerable modifications have been described for the improvement of both diagnostic methods. The technical complexity of the former furnished an incentive for the development of the latter. Yet, des...

tionally collected and kept for some time, is a very poor control. Some positive sera give a complete fixation of the usual one or more MHD of the complement in a 1 in 200 dilution; others can do so in 1 in 5 dilution only. The margin.of variation allowed for in this control, 5 to 200, is too wide to be useful in indicating whether the antigen-antibody systems involved in the reaction of comple...

1. A specific complement fixation reaction test for Plasmodium knowlesi malaria in rhesus monkeys is reported with details involved in the preparation of the antigen and procedures employed in setting up the test. 2. It was found that specific complement-fixing antibodies appeared early in the course of the experimental disease and persisted during the course of the chronic infection. 3. The fi...

Japanese encephalitis virions contain a 58 X 10-3-molecular-weight envelope glycoprotein antigen that can be solubilized with sodium lauryl sulfate and separated from other virion structural polypeptides and viral ribonucleic acid by gel filtration chromatography. The 58 X 10-3-molecular-weight envelope protein is the major antigen responsible for cross-reactivity of the virion in complement fi...