The accumulation of trehalose is a critical determinant of stress resistance in the yeast Saccharomyces cerevisiae. We have constructed a yeast strain in which the activity of the trehalose-hydrolyzing enzyme, acid trehalase (ATH), has been abolished. Loss of ATH activity was accomplished by disrupting the ATH1 gene, which is essential for ATH activity. The delta ath1 strain accumulated greater...

In Arc welding heat is transferred to the joint by an electrode. During manual metal Arc Welding this heat is transferred by melting the electrode. The stresses generated in welded plate are due to temperature raise and can be simple and thermal stresses. The stresses generated will cause distortion and changing the shape of the welded plates. The study of the distortion and residual stresses i...

OBJECTIVES To find evidence, either corroborating or refuting, for many persisting beliefs regarding the feasibility of carrying out surgical randomised controlled trials with a placebo arm, with emphasis on the challenges related to recruitment, funding, anaesthesia or blinding. DESIGN Systematic review. DATA SOURCES AND STUDY SELECTION The analysis involved studies published between 1959 ...

BACKGROUND Toxoplasma gondii is one of the most common causes of latent infections in humans worldwide. Detecting anti-Toxoplasma antibodies in serum using serological tests is a common method to diagnose toxoplasmosis. OBJECTIVES In the present study, an indigenous ELISA kit was prepared using tachyzoites from the RH strain of T. gondii, and its sensitivity and specificity were compared with...

Porcine reproductive and respiratory syndrome (PRRS) is endemic in most pork producing countries. In Chile, eradication of PRRS virus (PRRSV) was successfully achieved in 2009 as a result of the combined efforts of producers and the animal health authorities. In October 2013, after several years without detecting PRRSV under surveillance activities, suspected cases were confirmed on a commercia...

BACKGROUND The autotransporter (AT) system can potentially be used in the secretion of saccharolytic enzymes for the production of lignocellulosic biofuels and chemicals using Escherichia coli. Although ATs share similar structural characteristics, their capacity for secreting heterologous proteins widely varies. Additionally, the saccharolytic enzyme selected to be secreted should match the ce...

Ž . Axenic rotifers Brachionus plicatilis Muller were cultured under aseptic conditions; they ̈ Ž . were fed either a bacteria-free artificial diet AD , or axenic Isochrysis galbana, or a combination of axenic Chlorella minutissima and the bacteria-free AD. The medium was inoculated with commercial bacterial additives or cultured strains of marine bacteria. The highest improvements in Ž . growth...

Glucose oxidase (GOx) has found wide range of industrial applications. Commercial GOx are produced from Aspergillus niger and selected strains of Penicllium species, however, these strains are associated with some drawbacks. Aspergillus niger produces predominantly intracellular GOx, which requires extra recovery processes thus incurs more processing cost while Penicillium sp. exhibits filament...

Mixed strain cultures, used extensively for the manufacture of certain dairy products, have generally been thought to give a measure of protection against possible serious retardation of acid development resulting from attack by bacteriophages. Yet little information is available as to the influence that continued propagation has on the individual strains of lactic streptococci. Nichols and Ine...