Although plasminogen purification methods based on cellulose column chromatography have been described (l-4), the procedure based on acid extraction of Cohn’s plasma Fraction III (5) has continued to be widely used because of its simplicity, reproducibility and the absence of a limitation on the amount of Fraction III which can be processed. With the Fraction III available in 1953, plasminogen ...

A modern apparatus for performing flash chromatography using commercially available, prepacked silica cartridges has been developed. The key advantage of this system, when compared to traditional flash chromatography, is its use of commercially available silica cartridges, which obviates the need for students to handle silica gel. The apparatus has been tested for its ability to perform separat...

A 20-kDa protein (p20) that had internal amino acid sequences highly similar to those of aB crystallin was purified from rat and human skeletal muscle. p20 coeluted with (YB crystallin and HSP27/28 during column chromatography on DEAE-Sepharose and on Bio-Gel A-5m. p20 was separated from aB crystallin and HSP27/28 and was resolved into two fractions, a minor first peak and a major second peak,...