Agar cultures of blood and marrow cells were used to determine the nature and frequency of granulocytic progenitor cells (in vitro colony-forming cells) in 133 patients with various hematologic diseases, including 33 with acute and 17 with chronic myeloid or myelomonocytic leukemia. Cultures from patients with acute myeloid leukemia (AML) in relapse or in the acute transformation phase of chron...

Two novel human beta-chemokines, Ck beta-8 or myeloid progenitor inhibitory factor 1 (MPIF-1), and Ck beta-6 or MPIF-2, were discovered as part of a large scale cDNA sequencing effort. The MPIF-1 and MPIF-2 cDNAs were isolated from aortic endothelium and activated monocyte libraries, respectively. Both of the cDNAs were cloned into a baculovirus vector and expressed in insect cells. The mature ...

Catalase incorporation into enumeration media caused a significant increase (greater than 63%) in the colony-forming abilities of airborne bacteria. Incubation for 30 to 60 min of airborne bacteria in collection fluid containing catalase caused a greater than 95% increase in colony-forming ability. However, catalase did not have any effects on enumeration at high relative humidities (80 to 90%).

The potential of hematopoietic stem cells (HSCs) from human immunodeficiency virus type-1 (HIV-1)-infected individuals, eg, self-renewal and multilineage differentiative capacity, might be perturbed due to the underlying disease. In this study, we assessed the HSC activity in the CD34+ Thy-1+ cell population of peripheral blood stem cells (PBSCs) of three asymptomatic HIV-1-infected individuals...

BACKGROUND Lung contusion (LC) followed by hemorrhagic shock (HS) causes persistent bone marrow (BM) dysfunction lasting up to 7 d after injury. Mesenchymal stem cells (MSCs) are multipotent cells that can hasten healing and exert protective immunomodulatory effects. We hypothesize that MSCs can attenuate BM dysfunction after combined LCHS. MATERIALS AND METHODS Male Sprague-Dawley rats (n = ...

Soft agarose culture human tumour colony forming assay for drug sensitivity testing: [3H]-Thymidine incorporation vs colony counting Summary In vitro drug sensitivity testing, both by optical colony counting and by a [3H]-TdR incorporation assay, was performed on human tumour cells proliferating in soft agar cultures. Cells from two different human tumour cell lines, 5 different human tumour xe...

The use of phytohemagglutinin-supplemented colony cultures has offered new opportunities recently for studying acute myeloid leukemia (AML) cell growth in vitro. The active stimulator cells for AML colony-forming cells have not been identified, although this could be important for optimal application of the technique and for elucidating differences in growth between normal and leukemic progenit...

In vitro colony-forming ability of untreated acute nonlymphocytic leukemia (ANLL) cells determined by the CFU-C assay with and without PHA presensitization, and also human Ia-like antigen of their surfaces were investigated. In vitro colony-forming ability of 32 ANLLs was classified into four types: (A) no colony growth: 16, (B) PHA-dependent colony growth: 9, (C) colony-stimulating factor (CSF...

Colony assays are now available to study erythroid differentiation at three different levels. Mixed hemopoietic colonies represent progeny of pluripotent progenitors (CFU-GEMM). Erythroid bursts and cobnies are derived from early (BFU-E) and late precursors (CFU-E) that are committed towards erythropoiesis. The three different types of colonies were examined for their content of fetal hemoglobi...

The sedimentation velocity profiles of the entities in mouse bone marrow responsible for erythropoietic burst formation (BFU-E) and for erythrocytic colony formation (CFU-E) have been studied under conditions designed to determine whether the values observed are real or result from cell interactions occurring during culture of the fractions. Bone marrow cells of adult C3Hf/Bi mice were subjecte...