نتایج جستجو برای: colony assay
تعداد نتایج: 273197 فیلتر نتایج به سال:
In this study, we investigated the role of interleukin-lp (IL-1s) in the malignant evolution of chronic myelogenous leukemia (CML) and the functional activity of IL-1 inhibitors. Bone marrow (BM) and peripheral blood (PB) low-density cells from 38 CML patients were studied in the colonyforming unit-granulocyte, erythrocyte, monocyte, megakaryocyte colony culture assay. Samples from patients wit...
Culture is the only reliable method available at present for the diagnosis of melioidosis. Though serological tests have been described, their value in routine diagnosis is controversial. All indirect immunofluorescent assay (IFA) was therefore evaluated to determine its use in the diagnosis of melioidosis. Whole cell antigen prepared from a laboratory isolate of Burkholderia pseudomallei was u...
OBJECTIVES CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis. METHODS Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony ...
The volume of crevicular fluid is directly proportional to the degree ofperiodontal inflammation. The Periopaper system, with readings taken using the Periotron, has gained wide acceptance in the literature for measuring this volume. Alternative methods have also been proposed. This study investigated an alternative device for measuring the volume and compared it to the readings taken in Periot...
We have devised a method of evaluation, in terms of Goldblatt units (GU), of the data obtained in various laboratories for the excretion of renin from canine and human kidneys. An average value of 1.3 X 10" GU/min g" normal renal tissue resulted for both dog and man. A blood pressure increase of 18—45 mm Hg was induced in normal or nephrectomized, conscious or anesthetized dogs by the continuou...
The nature of the colony-forming cell in the bone marrow of patients with monoclonal gammopathy, as defined in the stem cell assay described by Hamburger and Salmon, has been studied. It could be shown that the colony-forming cells produce immunoglobulins of the same idiotype, heavy chain and light chain, as the monoclonal bone marrow cells in the patient. Data regarding the presence or absence...
The clonogenic or colony formation assay is a widely used method to study the number and size of cancer cell colonies that remain after irradiation or cytotoxic agent administration and serves as a measure for the anti-proliferative effect of these treatments. Alternatively, this assay is used to quantitate the transforming potential of cancer associated genes and chemical agents. Therefore, th...
BACKGROUND Recent clinical studies have linked polymorphisms in the xeroderma pigmentosum group D (XPD) gene, a key repair gene involved in nucleotide excision repair, to increased risk of hepatocellular carcinoma (HCC). However, the cellular effects of XPD expression in cultured HCC cells remain largely uncharacterized. Therefore, the aim of this study was to characterize the in vitro cellular...
Esophageal cancer (EC) is the most lethal cancer, and it is of significant concern worldwide, particularly in China. However, there are no effective treatments to cure it, such as chemotherapy, surgery, or radiotherapy. This is attributed to the lack of understanding of the molecular mechanisms of EC. Recently, the superfamily of Jmj-containing KDMs has been shown to play an important role in t...
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