Discoidin domain receptor 2 (DDR2) is a fibrillar collagen receptor that is expressed in mesenchymal cells throughout the body. In the heart, DDR2 is selectively expressed on cardiac fibroblasts. We generated a germline DDR2 knockout mouse and used this mouse to examine the role of DDR2 deletion on heart structure and function. Echocardiographic measurements from null mice were consistent with ...

Cartilage oligomeric matrix protein (COMP) is a protein present in the cartilage matrix and is expressed more abundantly in osteoarthritis cartilage than in healthy cartilage. The present study was designed to investigate the effect of transforming growth factor β (TGFβ) on COMP deposition and the influence of COMP on collagen biochemistry in a long-term 3-dimensional culture. Bovine chondrocyt...

PURPOSE Keratan sulfate proteoglycans (KSPGs) in the corneal stroma are believed to influence collagen fibrillar arrangement. This study was performed to investigate the fibrillar architecture of the corneal stroma in mice homozygous for a null mutation in the corneal KSPG, mimecan. METHODS Wild-type (n = 9) and mimecan-deficient (n = 10) mouse corneas were investigated by low-angle synchrotr...

Introduction: Skin aging is the result of many cellular dysfunctions over time particularly fibroblasts and keratinocytes. These could be decelerated by preventive effects some skin treatments such as Intradermal injections. NCTF 135HA has a polycomponent formulation designed to improve efficacy non-cross linked hyaluronic acid (as micro-filler) on function. Although been used aesthetic practit...

Collagen V is a minor component of the heterotypic I/III/V collagen fibrils and the defective product in most cases of classical Ehlers Danlos syndrome (EDS). The present study was undertaken to elucidate the impact of collagen V mutations on skin development, the most severely affected EDS tissues, using mice harboring a targeted deletion of the alpha2(V) collagen gene (Col5a2). Contrary to th...

Skeletal muscle tissue repairs itself by the activation of satellite cells, which are associated with the myofibers [1,2]. Activated satellite cells migrate to the site of injury and generate myoblasts, which eventually differentiate and fuse to each other or to damaged myofibers to restore muscle structure and function [3–5]. The satellite cells also have the capacity to replenish their number...

BACKGROUND The purpose of this study is to report the safety and efficacy of a new application of collagen cross-linking using a novel device to achieve predictable refractive myopic changes in virgin corneas. METHODS Four cases were treated with a novel device employing very high-fluence collagen cross-linking applied in a myopic pattern. Prior to treatment, riboflavin solution was applied t...

Chronic wounds, particularly diabetic ulcers, represent a main public health concern with significant costs. Ulcers often harbor an additional obstacle in the form of tunneled or undermined wounds, requiring treatments that can reach the entire wound tunnel, because bioengineered grafts are typically available only in a sheet form. While collagen is considered a suitable biodegradable scaffold ...

No author has a financial or proprietary interest in any material or method mentioned. Abstract Aim: To study the distribution and predominant orientations of Type 1 collage n at different depths throughout the entire thickness of the human cornea. This information will form the basis of a full three-dimensional reconstruction of corneal lamellae prefe rred orientations. Methods: Femtosecond la...

INTRODUCTION Within the last decades, collagen types I and III have been established as a sufficient biomaterial for GBR and GTR procedures. They might also be an adequate matrix for soft tissue augmentations. However, collagen materials differ significantly regarding resorption time, biodegradation pattern and the invasion of inflammatory cells.The aim of the present study was to compare the b...