Thesis Abstract Several cytokines are known to be involved in the pathogenesis of non-Hodgkin Lymphomas (NHL). Given importance understanding genetic predisposition NHL, this work was designed study impact IL-10 (-1082 G/A; rs1800896 and -819 C/T; rs1800871), IL-6 (-174 G/C; rs1800795) CD38 (184C/G; rs6449182) gene polymorphisms on susceptibility Egyptians NHL. Both loci IL10 were genotyped usi...

Objective: To perform comparative diagnostic study with rotavirus infected stool samples for efficacy ofindigenously prepared molecular transport medium (IMTM).Study Design: It was an experimental study.Place and Duration of Study: The conducted at Department Microbiology University Karachi,Karachi Pakistan from June to July 2021.Materials Methods: current based on hospital surveillance rotavir...

Aim A spesifi c and rapid diagnosis such as RT-PCR asay is the most needed to minimize transmission of HIV-1 infection. Therefore, in this study we developed the RT-PCR assay that was spesifi c against the gag gene of HIV-1. Methods The developed RT-PCR assay was evaluated against 46 specimens that were obtained from voluntary counseling and testing for HIV (VCT) in Rumah Sakit Umum Pemerintah ...

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

in this study 130 bulk tank milk samples which were delivered to the pegah pasturisation factory inmashhad were collected randomly during the summer months. samples were firstly enriched in modifiedtrypticase soy broth containing novobiocin, followed by plating onto sorbitol macconkey agar supplementedwith cefixime and potassium tellurite for isolation of escherichia coli o157:h7. consequently ...

causes human intestinal cyclosporiasis. It is more common in the immunocompromised patients and mainly seen people living with HIV/AIDS (PLHA), post-renal transplant (PRT) children (IC). Diagnostic microscopy for oocysts of parasite less sensitive, requiring examination multiple stool samples. Here we developed a new single run polymerase chain reaction (PCR) assay detection it was used to know...

BACKGROUND In this study, we evaluated the analytical performance and clinical potential of a one-step multiplex real-time PCR assay for the simultaneous detection of 14 types of respiratory viruses using the AdvanSure RV real-time PCR Kit (LG Life Sciences, Korea). METHODS Three hundred and twenty clinical specimens were tested with the AdvanSure RV real-time PCR Kit and conventional multipl...

BACKGROUND Limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of Leishmania infection. Nowadays, real-time PCR is being increasingly applied to quantify infectious agents. In the present study, a real-time PCR assay was developed to estimate parasite burdens in lymph nodes of Leishmania major infected BALB/C mice. Enumer...

A new mixed-valence one-dimensional coordination polymer of formula {[CoII(MeOH)2][(μ-NC)2CoIII(dmphen)(CN)2]2}n·2nH2O (1) was obtained by reacting the Ph4P[CoII(dmphen)(CN)3] metalloligand (dmphen = 2,9-dimethyl-1,10-phenanthroline and Ph4P+ tetraphenylphosphonium ion) with cobalt(II) acetate tetrahydrate. The structural analysis shows formation a neutral 4,2-ribbon-like chain vertex-sharing c...

Pneumocystis jirovecii is an opportunistic pathogen in immunocompromised and AIDS patients. Detection by quantitative PCR is faster and more sensitive than microscopic diagnosis yet requires specific infrastructure. We adapted a real-time PCR amplifying the major surface glycoprotein (MSG) target from Pneumocystis jirovecii for use on the new BD MAX platform. The assay allowed fully automated D...