Introduction. Coagulase-negative staphylococci have been recognized both as emerging pathogens and contaminants of clinical samples. High-resolution genomic investigation may provide insights into their significance. Aims. To review the literature regarding coagulase-negative staphylococcal infection utility methods to aid diagnosis management, identify promising areas for future research. Meth...

We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagulase-negative Staphylococcus were, respectively, 100% and 98.3%, 100% and 100%, ...

Abstract Aim To compare the microbiology organisms responsible for PJI in patients who underwent two-stage revision of infected primary knee replacement with oncological endoprostheses lower limb a single institution. Method 118 were treated 2-stage surgery arthroplasty and between 1999 2019. 74 had 44 to eradicate infection tumour resection. All microbiological data from first stage sampling w...

Eighty clinical specimens of BACTEC 9240 blood culture vials, culture positive for staphylococci (38 Staphylococcus aureus and 42 coagulase negative staphylococci), were tested directly for the presence of clumping factor/protein A and free coagulase. Seven commercial slide agglutination kits were compared with a direct-tube coagulase (DTC) method. All tests were performed on blood culture pell...

Three commercially available rapid slide agglutination tests for the identification of Staphylococcus aureus were evaluated with 354 recent clinical isolates (165 strains of S. aureus). The test results of two latex agglutination products, SeroSTAT Staph (Scott Laboratories, Inc.) and Staphylatex (American Micro Scan), and one hemagglutination product, Staphyloslide (BBL Microbiology Systems), ...

The detection of thermonuclease activity in 86 blood culture samples containing gram-positive cocci showed 100% correlation with the subsequent identification of the isolate as Staphylococcus aureus by the coagulase test. No positive thermonuclease results were found with 66 samples containing coagulase-negative staphylococci and 56 samples containing other gram-positive organisms. The thermonu...

A procedure was developed for restriction endonuclease fingerprinting (REF) of the chromosomal DNA of coagulase-negative staphylococci. A total of 48 isolates comprising 29 Staphylococcus epidermidis and 19 Staphylococcus haemolyticus isolates from blood and mucocutaneous sites of 15 premature neonates were characterized by REF, plasmid profile (PP) analysis, antimicrobial susceptibility testin...

AIM To determine the etiology of peritonitis, as well as the sensitivity of isolated bacteria to antibiotics. METHODS Study was performed at the Surgical Clinic, Department of Anesthesiology and Reanimation, Department of Microbiology, Department of Clinical Pharmacology of University Clinical Centre (UCC) Tuzla. In a prospective study, starting from 1.6.2009. to 30.6.2010., was 60 patients a...

ISSN: 2319-7706 Volume 4 Number 5 (2015) pp. 172-190 http://www.ijcmas.com Neonatal sepsis is a leading cause of neonatal mortality in developing countries. Identification of the etiological agents of neonatal sepsis is essential for effective treatment. Out of 106 microbial isolates recovered from blood cultures of late onset neonatal sepsis (LONS) patients, only 70 (66 %) isolates were Gram-p...