UNLABELLED Confocal laser scanning microscopy (CLSM) is a powerful tool for investigation of biofilms. Very few investigations have successfully quantified concurrent distribution of more than two components within biofilms because: 1) selection of fluorescent dyes having minimal spectral overlap is complicated, and 2) quantification of multiple fluorochromes poses a multifactorial problem. O...

BACKGROUND Bacteria which are metabolically active yet unable to be cultured and eradicated by antibiotic treatment are present in the middle ear effusion of children with chronic otitis media with effusion (COME) and recurrent acute otitis media (rAOM). These observations are suggestive of biofilm presence or intracellular sequestration of bacteria and may play a role in OM pathogenesis. The a...

This work investigates bituminite (amorphous sedimentary organic matter) in Upper Jurassic Kimmeridge Clay source rock via confocal laser scanning microscopy (CLSM) and atomic force (AFM). These petrographic tools were used to provide better understanding of the nature bituminite, which has been historically difficult identify differentiate from similar matter types rocks. As part an Internatio...

Due to its particularity and importance, long-distance oil gas pipelines need be well protected from damage by backfill materials. In this study, construction waste expanded polystyrene (EPS) were used replace conventional fine aggregate, ethylene vinyl acetate-resin (EVA) was modify the surface of EPS prepare lightweight controlled low strength materials (CLSM). Lightweight CLSM tested in mech...

Coupled level set and CFD (computational fluid dynamics) methods are adopted in this work to track the moving gas–liquid interfaces riser of an external loop airlift photobioreactor (ALR) which microalgae used produce biofuels capture CO2 from flue-gas. Modeling behavior gas bubbles is a crucial aspect for fine-tuning operation reactor when inserted into closed-loop biorefinery at pilot-scale. ...

Zusammenfassung Hintergrund und Ziele Die ex‐vivo konfokale Laserscanmikroskopie (CLSM) erlaubt die feingewebliche Untersuchung von Nativpräparaten innerhalb Minuten basierend auf Gewebereflexion fluoreszenzbasierter Kernfärbung. neu eingeführte digitale Farbbearbeitung der Bilder imitiert konventionelle Hämatoxylin‐Eosin (HE)‐Färbung nahezu perfekt. Ziel war eine Evaluation neuen Methode im Ze...

Understanding the exchange of energy between surface and atmosphere is important in view climate scenario. However, it becomes a challenging task due to sparse network observations. This study aims improve balance estimates for Amazon, Cerrado, Pampa biomes located South America using radiation precipitation forcing obtained from Clouds Earth’s Radiant Energy System (CERES) CPTEC/MERGE datasets...