A modified slide culture method which combines advantages found in several slide culture methods is described. A block of inoculated nutrient agar sandwiched between two sterile cover glasses is placed in a plastic petri dish containing water agar. After adequate growth has occurred, the slide culture is disassembled and mounted in a conventional manner.

edigraphic.com Blastocystis hominis is a strict anaerobic protozoan that occurs in the intestine of humans and other mammals (Zierdt, 1991). Recently, phylogenetic analyses of ribosomal RNAs place B. hominis within the straminopiles (Silberman et al., 1996). Its pathogenic role in human beings is still disputed by some investigators (Markell and Udkow, 1986; Miller, 1988). The mechanism by whic...

The main objective of the recent work in this laboratory on typhus fever has been the development of a method by which it might be possible to obtain large amounts of virulent Rickettsia for purposes of vaccine production. I t has been relatively easy to obtain enormous yields of the murine type of Rickettsia by the x-ray rat method, a technique, previously discussed (1), which has been in week...

The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been dev...

The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been dev...

The main objective of the recent work in this laboratory on typhus fever has been the development of a method by which it might be possible to obtain large amounts of virulent Rickettsia for purposes of vaccine production. I t has been relatively easy to obtain enormous yields of the murine type of Rickettsia by the x-ray rat method, a technique, previously discussed (1), which has been in week...