Affinity chromatography uses biospecific binding usually between an antibody and an antigen, an enzyme and a substrate or other pairs of key-lock type of matching molecules. Due to its high selectivity, it is able to purify proteins and other macromolecules from very dilute solutions. In this work, a general rate model for affinity chromatography was used for scale-up studies. Parameters for th...

The affinity ligand quality of the triazine dye, cibacron blue, toward serum albumin has been well studied [64-68]. By immobilizing the ligand to a stationary phase, affinity separations can be performed in order to remove bulk albumin from serum samples. This decrease in albumin concentration may serve as a direct advantage for proteomics as large amounts of serum albumin hinder the study of l...

In an attempt to systematically dissect the ligand binding properties of human serum albumin (HSA), the gene segments encoding each of its three domains were defined based on their conserved homologous structural motifs and separately cloned into a secretion vector for Pichia pastoris. We were able to establish a generally applicable purification protocol based on Cibacron Blue affinity chromat...

The interaction effect of eight operating factors on the degradation three organic dyes different structures (Cibacron green, methylene blue and methyl orange) has been studied. Effect had evaluated statistically using Plackett-Burman screening design which ex-tracted valuable information most important parameters their interactions. goodness model fit was checked by determination coefficient R...

Xylitol dehydrogenase (EC1.1.1.9) from xylose-grown cells of Candida shehatae was purified 215-fold by sequential chromatography on NADC8 affinity, Superose-12, and Cibacron blue columns, and a single band was observed by SDS gel electrophoresis. The purified enzyme had a native molecular weight of 82 kDa and a denatured molecular weight of 40 kDa following SDS gel electrophoresis, indicating t...

For a successful detection of gravitational waves by LISA, it is essential to construct theoretical waveforms in a reliable manner. We discuss gravitational waves from an extreme mass ratio binary system which is expected to be a promising target of the LISA project. The extreme mass ratio binary is a binary system of a supermassive black hole and a stellar mass compact object. As the supermass...

Several recent observational studies have shown organic carbon aerosols to be a significant source of absorption of solar radiation. The absorbing part of organic aerosols is referred to as “brown” carbon (BrC). Using a global chemical transport model and a radiative transfer model, we estimate for the first time the enhanced absorption of solar radiation due to BrC in a global model. The simul...

We established experiments on farmers’ fields at four locations in southcentral Alaska and two locations in interior Alaska. The southcentral Alaska sites were near Anchor Point (59 ̊45'N, 151 ̊39'W) on Edna Anderson’s farm, near Sterling (60 ̊32'N, 150 ̊39'W) on Paul Rumley’s farm, at Point Mackenzie (61 ̊24'N, 150 ̊04'W) on the Trytten Farm, and at Palmer (61 ̊39'N, 150 ̊04'W) on Peter Scorup’s farm ...