نتایج جستجو برای: chitin binding domain

تعداد نتایج: 765299  

2017
Ka H. Wong Wei Liang Tan Shruthi G. Kini Tianshu Xiao Aida Serra Sui Kwan Sze James P. Tam

Hevein and hevein-like peptides are disulfide-constrained chitin-binding cysteine-rich peptides. They are divided into three subfamilies, 6C-, 8C-, and 10C-hevein-like peptides, based on the number of cysteine residues. In addition, hevein-like peptides can exist in two forms, short and long. The long C-terminal form found in hevein and 10C-hevein-like peptides contain a C-terminal protein carg...

Journal: :The Journal of biological chemistry 2002
Fabrizia Fusetti Holger von Moeller Douglas Houston Henriette J Rozeboom Bauke W Dijkstra Rolf G Boot Johannes M F G Aerts Daan M F van Aalten

Chitin hydrolases have been identified in a variety of organisms ranging from bacteria to eukaryotes. They have been proposed to be possible targets for the design of novel chemotherapeutics against human pathogens such as fungi and protozoan parasites as mammals were not thought to possess chitin-processing enzymes. Recently, a human chitotriosidase was described as a marker for Gaucher diseas...

2014
Lei Chen Tian Liu Yong Zhou Qi Chen Xu Shen Qing Yang

Insects possess a greater number of chitinases than any other organisms. This work is the first report of unliganded and oligosaccharide-complexed crystal structures of the insect chitinase OfChtI from Ostrinia furnacalis, which is essential to moulting. The obtained crystal structures were solved at resolutions between 1.7 and 2.2 Å. A structural comparison with other chitinases revealed that ...

Journal: :The Journal of biological chemistry 1994
S S Sidhu G B Kalmar L G Willis T J Borgford

In this report we describe a novel chymotrypsin-like serine protease produced by Streptomyces griseus. The enzyme has been tentatively named S. griseus protease C (SGPC). The gene encoding the enzyme (sprC) was identified and isolated on the basis of its homology to the previously characterized S. griseus protease B (SGPB). The sprC gene encodes a 457-amino acid prepro-mature protein of which o...

Journal: :Phytochemistry 2003
Ok Sun Lee Boyoung Lee Nammi Park Ja Choon Koo Young Hoe Kim Theertha Prasad D Chandrakant Karigar Hyun Jin Chun Byoung Ryong Jeong Doh Hoon Kim Jaesung Nam Jae-Gil Yun Sang-Soo Kwak Moo Je Cho Dae-Jin Yun

The antifungal activity of hevein-like proteins has been associated with their chitin-binding activities. Pn-AMP1 and Pn-AMP2, two hevein homologues from Pharbitis nil, show in vitro antifungal activities against both chitin and non-chitin containing fungi. Purified Pn-AMPs retained antifungal activities only under non-reducing conditions. When Pn-AMP2 cDNA was constitutively expressed in tomat...

Journal: :Current allergy and asthma reports 2014
Wayne R Thomas

As investigations into the innate immune responses that lead to allergic sensitization become better defined, there is a need to determine how allergens could interact with pattern recognition receptors that bind non-proteinaceous moieties. Many important allergens are not covalently bound to lipid or carbohydrate, but have structures belonging to lipid, glycan and glycolipid-binding families. ...

Journal: :Glycobiology 2011
Tanja Larsen Bent O Petersen Birgit G Storgaard Jens Ø Duus Monica M Palcic Jørgen J Leisner

Salmonella contain genes annotated as chitinases; however, their chitinolytic activities have never been verified. We now demonstrate such an activity for a chitinase assigned to glycoside hydrolase family 18 encoded by the SL0018 (chiA) gene in Salmonella enterica Typhimurium SL1344. A C-terminal truncated form of chiA lacking a putative chitin-binding domain was amplified by PCR, cloned and e...

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