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In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating the bulk cell density (BCD: cell number per culture volume) deterministically alters anteroposterior patterning of primitive streak (PS)-like priming. The BCD in conjunction with the c...
To harness the potential of human pluripotent stem cells (hPSCs), an abundant supply of their progenies is required. Here, hPSC expansion as matrix-independent aggregates in suspension culture was combined with cardiomyogenic differentiation using chemical Wnt pathway modulators. A multiwell screen was scaled up to stirred Erlenmeyer flasks and subsequently to tank bioreactors, applying control...
Revenant sur la séquence étatsunienne qui a récemment conduit à une politisation intense de Critical Race Theory (CRT), l’article propose revisiter l’histoire relativement récente et les hypothèses fondatrices ce corpus académique. Avant tisser des liens avec sociologie du racisme, telle qu’on lit en France, sous plume Colette Guillaumin. L&#...
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