1. A strain of meningococci obtained directly from the spinal fluid of a patient has been propagated in serial passage in 10 to 12 day old chick embryos without change in its essential characteristics. 2. The chick embryo is susceptible to infection with the meningococcus, and, depending on its stage of development, reacts to the infection with more or less specific lesions. 3. In chick embryos...

1. Radioisotope incorporation studies of normal and lathyritic chick embryo bone collagen do not demonstrate any interference by lathyrism with collagen synthesis or fibril formation. 2. The results indicate that a portion of the extractable collagen from lathyritic chick embryo bone represents newly synthesized protein. Evidence from a double labeling experiment and from analysis of isotope fl...

In previous experiments, we have demonstrated that limb buds engrafted during embryonic life at E4, between MHC-mismatched chick embryos, are not only tolerated after birth, but induce in the recipient a state of split tolerance toward cells expressing the donor MHC haplotype: donor's skin grafts are permanently tolerated while a proliferative response of host's T cells is generated in MLR by d...

1. Suspensions of isolated chick jejunal columnar absorptive (brush-border) cells respired on endogenous substrates at a rate 40% higher than that shown by rat brush-border cells. 2. Added d-glucose (5 or 10mm), l-glutamine (2.5mm) and l-glutamate (2.5mm) were the only individual substrates which stimulated respiration by chick cells; l-aspartate (2.5 or 6.7mm), glutamate (6.7mm), glutamine (6....

Stocks of three Indian Chandipura virus (CHPV) isolates; one isolate from an adult febrile case in 1965 from Chandipura town, Maharashtra, and two isolates from two pediatric encephalitis cases from Andhra Pradesh, 2003 were inoculated in 10-day-old chick embryos by allantoic route. All three virus isolates replicated in chick embryos showing titre of log 10(12) to log 10(13) EID50. The results...

German embryologist Viktor Hamburger [4] came to the US in 1932 with a fellowship provided by the Rockefeller Foundation [5]. Hamburger started his research in Frank Rattray Lillie?s laboratory at the University of Chicago [6]. His two-year work on the development of the central nervous system [7] (CNS) in chick [8] embryos was crystallized in his 1934 paper, ?The Effects of Wing Bud Extirpatio...

We have isolated the chick and mouse homologs of human aldehyde dehydrogenase 6 (ALDH6) that encode a third cytosolic retinaldehyde-specific aldehyde dehydrogenase. In both chick and mouse embryos, strong expression is observed in the sensory neuroepithelia of the head. In situ hybridization analysis in chick shows compartmentalized expression primarily in the ventral retina, olfactory epitheli...

A chick brain-derived growth factor (CBGF) has been purified to apparent homogeneity by a combination of ultrafiltration, DEAE ion-exchange, and reversed-phase high-performance liquid chromatography on a C18 column. CBGF has an apparent molecular mass of approximately 1500 Da. The amino acid composition of CBGF has been determined and reveals a peptide containing predominantly glycine, glutamic...

Evidence was obtained indicating differences in the survival rate of Western equine encephalomyelitis virus after exposure to ultraviolet radiation and methyl methanesulfonate in commercial and leukosisfree chick embryo cells that differed in repair activity. The levels of spontaneous mutagenesis (on the basis of the yield of small palque variants of the encephalomyelitis virus) did not essenti...

The blood-brain barrier is characterized by still poorly understood barrier and transport functions performed by specialized endothelial cells. Hybridoma technology has been used to identify a protein termed neurothelin that is specific for these endothelial cells. Neurothelin is defined by the species-specific mouse mAb lW5 raised against lentil-lectin-binding proteins of neural tissue from em...