objective:at the present study, relationship between phagocytosis of plga particles and combined effects of particle size and surface pegylation was investigated.  materials and methods:microspheres and nanospheres (3500 nm and 700 nm) were prepared from three types of plga polymers (non-pegylated and pegylation percents of 9% and 15%). these particles were prepared by solvent evaporation metho...

objective(s) motility plays a major role in pathogenicity of helicobacter pylori, yet there is  scarce data regarding its chemotactic behaviour. the present study was designed to investigate the chemotactic responses of local isolates of h. pylori towards various sugars, amino acids, as well as some other chemical substances. materials and methods chemotaxis was assayed by a modified adler’s me...

In this work, we rigorously study chemotaxis effect versus haptotaxis on boundedness, blow-up and asymptotical behavior of solutions for a chemotaxis-haptotaxis model in 2D settings. It is well-known that the corresponding Keller–Segel chemotaxis-only possesses striking feature critical mass blowup phenomenon, namely, subcritical ensures whereas, supercritical induces existence blow-ups. Herein...

Neutrophils play an essential role in the innate immune response to microbial infection and are particularly important in clearing bacterial infection. We investigated the role of the transcription factor FOXO1 in the response of neutrophils to bacterial challenge with Porphyromonas gingivalis in vivo and in vitro. In these experiments, the effect of lineage-specific FOXO1 deletion in LyzM.Cre+...

Posttranslational modifications (PTMs) of cytoskeleton proteins due to oxidative stress associated with several pathological conditions often lead to alterations in cell function. The current study evaluates the effect of nitric oxide (DETA-NO)-induced oxidative stress-related S-glutathionylation of cytoskeleton proteins in human PMNs. By using in vitro and genetic approaches, we showed that S-...

Dictyostelium discoideum cells harbor two annexin VII isoforms of 47 and 51 kDa which are present throughout development. In immunofluorescence and cell fractionation studies annexin VII was found in the cytoplasm and on the plasma membrane. In gene disruption mutants lacking both annexin VII isoforms growth, pinocytosis, phagocytosis, chemotaxis and motility were not significantly impaired und...

Alterations in neutrophil subpopulations during human hemodialysis or following injection of C5a,,,, into rabbits were studied. Whereas baseline peripheral blood neutrophils contained approximately 80% of cells that formed rosettes with lgG-sensitized erythrocytes. neutrophils harvested at the granulocyte nadir (20 mm after initiating hemodialysis or the injection of C5a,, ,, arg) were markedly...

To stimulate granulopoiesis, we gave recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF; 120 microgram/m2/d) to a patient with congenital neutropenia. The treatment resulted in marked increases in white blood cell counts (maximum, 17,400/microL), consisting mainly of eosinophils (maximum, 13,050/microL) and monocytes (maximum, 1305/microL), rather than neutrophils (maxim...