نتایج جستجو برای: chemoenzymatic reaction

تعداد نتایج: 413126  

2016
Renpeng Liu John Giddens Colleen M. McClung Paula E. Magnelli Lai-Xi Wang Ellen P. Guthrie

Glycosylation affects the efficacy, safety and pharmacokinetics/pharmacodynamics properties of therapeutic monoclonal antibodies (mAbs), and glycoengineering is now being used to produce mAbs with improved efficacy. In this work, a glycoengineered version of rituximab was produced by chemoenzymatic modification to generate human-like N-glycosylation with α 2,6 linked sialic acid. This modified ...

Journal: :ACS chemical biology 2012
Go Sugiarto Kam Lau Jingyao Qu Yanhong Li Sunghyuk Lim Shengmao Mu James B Ames Andrew J Fisher Xi Chen

Glycosyltransferases are important catalysts for enzymatic and chemoenzymatic synthesis of complex carbohydrates and glycoconjugates. The glycosylation efficiencies of wild-type glycosyltransferases vary considerably when different acceptor substrates are used. Using a multifunctional Pasteurella multocida sialyltransferase 1 (PmST1) as an example, we show here that the sugar nucleotide donor h...

2016
Shubing Tang Baoqin Xuan Xiaohua Ye Zhong Huang Zhikang Qian

Virus-like particles (VLPs) can be used as powerful nanoscale weapons to fight against virus infection. In addition to direct use as vaccines, VLPs have been extensively exploited as platforms on which to display foreign antigens for prophylactic vaccination and immunotherapeutic treatment. Unfortunately, fabrication of new chimeric VLP vaccines in a versatile, site-specific and highly efficien...

2016
Kenjiro Yazawa Keiji Numata Katja Loos

Peptides have the potential to serve as an alternative for petroleum-based polymers to support a sustainable society. However, they lack thermoplasticity, owing to their strong intermolecular interactions. In contrast, nylon is famous for its thermoplasticity and chemical resistance. Here, we synthesized peptides containing a nylon unit to modify their thermal properties by using papain-catalyz...

Journal: :Bioscience, Biotechnology, and Biochemistry 1994

Journal: :Chemical and Pharmaceutical Bulletin 2006

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