Genetic studies provide valuable data to inform conservation strategies for species with small or declining populations. In these circumstances, obtaining DNA samples without harming the study organisms is highly desirable. Excrements are increasingly being used as a source of DNA in such studies, but such approaches have rarely been applied to arthropods. Bumblebees are ecologically and econom...

AIM To establish allele frequencies and genetic parameters for 5 new European Standard Set short tandem repeat (STR) loci in the population of Romania and to compare them with those in other populations. METHODS DNA was isolated using QIAamp 96 DNA Swab BioRobot Kit and Chelex 100 methods. Polymerase chain reaction amplification was done using Investigator ESSplexPlus Kit (D1S1656, D2S441, D2...

13 The dynamic technique of diffusive gradients in thin films (DGT), that measures metal 14 speciation in situ, has found wide environmental application. Simple interpretation of the 15 metal accumulation in terms of a solution concentration has assumed that trace metals do 16 not penetrate beyond the surface of the binding layer, but penetration, although 17 theoretically discussed has not yet...

This paper describes the equilibrium behaviour of a wastewater resembling that produced during semiconductor and printed circuit board manufacture when contacted with a chelating ion exchange resin (Chelex-100; Bio-Rad Laboratories). Distribution coefficients (solid phase metal concentrations/soluble metal concentration) were calculated, and it was determined that the order of affinity was Cu >...

The influence of Zn on the expression of the apolipoprotein A-I (apoA-I) gene in Hep G2 cells was examined. Zn depletion was achieved with a low-Zn (ZD) medium prepared from Zn-free growth medium (Opti), a ZD medium containing Chelex 100-extracted fetal bovine serum (CHE), and a medium containing chelator 1,10-phenanthroline (OP). Compared with those for their respective controls, cellular Zn l...

OBJECTIVES This preliminary study was carried out to determine the allele frequencies and forensic efficiency parameters for the short tandem repeat loci CSF1PO, TPOX, THO1, D16S539, D7S820, D13S317, vWA, FESFPS and F13B in a test sample population of Sri Lankans. DESIGN Test samples were obtained from 305 non-related individuals originating from all 9 provinces of Sri Lanka. DNA was extracte...

This paper deals briefly with a technique developed for the identification of the early stages of veligers of the surfclam Spisula solidissima from the larval stages of other common bivalve species using a combination of DNA probe and restriction fragment length polymorphism (RFLP) analysis. An oli¬ gonucleotide sequence designed from the 18S ribosomal RNA gene (nucleotide position 259-276) pro...

AIM To determine allele frequencies' distribution for 15 short tandem repeat (STR) loci in a population sample of 1910 unrelated individuals from the region of Wallachia, South Romania. METHODS DNA was isolated using Chelex 100 method and an adapted version of AGOWA mag DNA Isolation Kit Sputum. Polymerase chain reaction amplification was done using AmpFlSTR Identifiler kit (D8S1179, D21S11, ...

Korean native honey (KNH) is much more expensive than European honey (EH) in Korea, because KNH is a favored honey which is produced less than EH. Food fraud of KNH has drawn attention of the government office concerned, which is in need of a method to differentiate between KNH and EH which are produced by the Asiatic honeybee, Apis cerana and the European honeybee, Apis mellifera, respectively...

The extraction of soil organic phosphorus by the NaOH-EDTA procedure was assessed in detail for a tropical forest soil (clay-loam, pH 4.3, total carbon 2.7%). Optimum conditions for the quantification of soil organic phosphorus and characterization of its composition by solution P NMR spectroscopy were extraction in a solution containing 0.25 M NaOH and 50 mM Na2EDTA in a 1:20 solid to solution...