Zonal centrifugation, based either on sediInentation rate or on isopycnic banding in liquid density gradients, is useful for high-resolution separation of subcellular particles. However, the amoun t of material which may be separated is extremely limited when conventional centrifuges are employed. To increase the capacity and, in certain instances, the resolution of the method, the possibility ...

Fungemia is associated with high morbidity and mortality rates. Various techniques have been introduced to diagnose fungemia such as conventional broth culture medium, by blind or macroscopic subculture, vented and aerated biphasic culture medium, lysis Þ ltration agitated culture medium, radiometric and nonradiometric methods, lysis centrifugation method, etc..[1] Of these, lysis-centrifugatio...

To characterize the desmosome components that mediate intercellular adhesion and cytoskeletal-plasma membrane attachment, we prepared whole desmosomes and isolated desmosomal intercellular regions (desmosomal "cores") from the living cell layers of bovine muzzle epidermis. The tissue was disrupted in a nonionic detergent at low pH, sonicated, and the insoluble residue fractionated by differenti...

Specimen centrifugation is one of the most time-consuming tasks associated with preanalytical specimen processing. We report the first clinical use and impact study of the novel centrifugation method, axial separation, using the Axial Separation Module (ASM). The ASM centrifuges medical specimens one at a time (in 1 min 9 s) in proprietary Axial Process Containers (APC). Here we report tests of...