Statistical experimental design was applied for the optimization of medium constituents for citric acid production by Yarrowia lipolytica NCIM 3589 in submerged fermentation using raw glycerol as the carbon source. Response surface methodology (RSM) involving central composite design (CCD) was adopted to evaluate the amount of citric acid produced by most important factors, such as carbon conce...

A statistical design approach has been used to optimize the production of biomass by Cunninghamella sp. 2A1, evaluated based on lipidless biomass. A 2 3 full factorial central composite design (CCD) was chosen to study the combined effects of three factors; ammonium tartrate, peptone and glucose concentrations. The p-value for each factor was <0.05 suggesting that these factors have significant...

Insects are a readily available source of chitosan due to their high reproductive rates, ease breeding, and resistance changes in ecosystem. This study aimed extract from several widespread insects: Blaps lethifera (CS-BL), Pimelia fernandezlopezi (CS-PF), Musca domestica (CS-MD). The was also extended using the obtained chitosans removing methylene blue dye (MB) wastewater. chitosan, initial c...

The conventional solvent extraction (CSE) method is commonly used to extract oil from oilseeds. Even though the of soybean seeds using CSE has been studied extensively, application response surface methodology (RSM) not yet widely explored. In this paper, was employed three different types solvents – ethanol (Eth), n-hexane (Hex), and a mixture Hex:Eth in ratio 2:1 optimized RSM Cambodian seeds...

among various heuristics techniques, genetic algorithm (ga) is one of the most widely used techniques which has successfully been applied on a variety of complex combinatorial problems. the performance of ga largely depends on the proper selection of its parameters values; including crossover mechanism, probability of crossover, population size and mutation rate and selection percent. in this p...

inulin is widely distributed throughout the plant kingdom. it has been increasingly used in various foods due to its beneficial nutritional attributes as prebiotic ingredient. the helianthus tuberosus could be a source of inulin, but scarce studies about its extraction exist. ultrasound has attracted considerable interest in food science and technology due to its promising effects in food proce...

Abstract The enzyme urate oxidase (UOX) is used as a drug for preventing and treatment of hyperuricemia. This study deals with the statistical optimization lactose inducible fermentation production soluble recombinant Aspergillus flavus UOX. 10 independent variables were investigated by Plackett–Burman design (PBD), most significant factors further optimized central composite (CCD). PBD results...

Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major factors (hydrolysis time, water volume and ethyl acetate volume) were screened out through Plack...

Arginine metabolism in Enterococcus faecium sp. GR7 was enhanced via arginine deiminase pathway. Process parameters including fermentation media and environmental conditions were optimized using independent experiments and response surface methodology (central composite design). Fermentation media (EAPM) were optimized using independent experiments which resulted in 4-fold increase in arginine ...

The aim of present work was to find out the optimum combination of extraction process variables of hypericin from St. John’s wort, by revealing the mathematical functional relation of extraction temperature, ethanol concentration for the extraction yield, and to develop a validated HPLC method to determine hypericin in St. John’s wort extracts prepared. The HPLC separation was performed on a Lu...