Pseudomonas putida KT2442 is a bacterium with potential for use in bioremediation of chlorinated hydrocarbons in soils, and its behavior in the subsurface is believed to be controlled by surface polymers. The role of surface macromolecules on the retention and adhesion behavior of this bacterium was examined by bacterial treatment with cellulase, an enzyme that breaks down the (1 4) linkages of...

The effects of different additives, including lactic acid bacteria inoculants, acremonium cellulase and mixtures of lactic acid bacteria inoculants and acremonium cellulase on changes of nutritive components and in vitro digestibility of Ceratoides arborescens during ensiling were investigated. The silages of each treatment were prepared in plastic film bags, kept in an incubator at 30°C and ba...

*Correspondence: Rongxin Su, State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China e-mail: [email protected] Despite the advantageous prospect of second-generation bioethanol, its final commercialization must overcome the primary cost impediment due to enzyme assumption. To solve this problem, this work achieves high...

In energy deficient world, cellulases play a major role for the production of alternative energy resources utilizing lignocellulosic waste materials for bioethanol and biogas production. This study highlights fungal and bacterial strains for the production of cellulases and its industrial applications. Solid State Fermentation (SSF) is more suitable process for cellulase production as compared ...

Aspergillus niger is an important microorganism that has been used for decades to produce extracellular enzymes. In this study, a novel Aspergillus niger strain integrated with a eukaryotic expression vector harboring the gpd-Shi promoter of shiitake mushrooms and cellulase gene of Ampullaria gigas Spix was engineered to improve cellulase production for the achievement of highly efficient sacch...

The adsorption of cellulases onto fibers may be one of the most important factors affecting the enzymatic reaction between cellulases and fibers. This study investigated the adsorption kinetics involved, using isothermal adsorption equations. Cellulose binding domains (CBDs) were isolated from a commercial cellulase, and their role in the adsorption and enzymatic reaction was evaluated. Approxi...

The rumen bacterium Cellulosilyticum ruminicola H1 efficiently hydrolyzes cellulose. To gain insights into the regulatory mechanisms of cellulase synthesis, comparative transcriptome analysis was conducted for cultures grown on 2% filter paper, 0.5 and 0.05% cellobiose, and 0.5% birchwood xylan. It was found that cellulose induced a majority of (hemi)cellulases, including 33 cellulases and a ce...

The cellulase producing bacteria was isolated from cow dung and characterized by morphological and biochemical analysis. Further partial purification of cellulase was carried out by dialysis and ammonium phosphate precipitation and also to determine molecular weight by SDS-PAGE. Bacterial isolated were grown on Carboxy Methyl Cellulose (CMC) agar at various optimum conditions such as parameters...

Lactose is the only soluble and economically feasible carbon source for the production of cellulases or heterologous proteins regulated by cellulase expression signals by Hypocrea jecorina (Trichoderma reesei). We investigated the role of the major -galactosidase of H. jecorina in lactose metabolism and cellulase induction. A genomic copy of the bga1 gene was cloned, and this copy encodes a 1,0...

A laboratory in situ composting study was conducted as a possible alternative method for the current practice of open air burning of post-harvest sugarcane residue by sugarcane farmers. In situ composting of the sugarcane residue by the indigenous bacteria and fungi was accelerated using molasses as an initial substrate. A one-time application of molasses boosted the soil microbial population. ...