Objective Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mor...

Stem/progenitor cells from multiple tissues have been isolated based on enhanced activity of cytosolic aldehyde dehydrogenase (ALDH) enzyme. ALDH activity has emerged as a reliable marker for stem/progenitor cells, such that ALDH(bright/high) cells from multiple tissues have been shown to possess enhanced stemness properties (self-renewal and multipotency). So far though, not much is known abou...

BACKGROUND Proper treatment of lymph node-negative breast cancer depends on an accurate prognosis. To improve prognostic models for this disease, we evaluated whether an immunohistochemical marker for proliferating cells, Ki-S2 (a monoclonal antibody that binds to a 100-kd nuclear protein expressed in S, G2, and M phases of the cell cycle), is an accurate indicator of prognosis. METHODS We st...

Background: Rhabdomyosarcoma is a rare malignant soft tissue sarcoma and the most common sarcoma of childhood. The purpose of this study was to investigate the distribution of age and sex of patients, pathological subtypes and to determine the role of staging and classification of RMS on treatment outcome. Materials and Methods: This retrospective study included patients who diagnosed with RMS ...

The use of a 3D perfusion culture environment for stem cell expansion has been shown to be beneficial for maintenance of the original cell functionality but due to several system inherent characteristics such as the presence of extracellular matrix, the continued development and implementation of 3D perfusion bioreactor technologies is hampered. Therefore, this study developed a methodology for...

The expression of activated ras genes has been implicated as a contributing factor to the radioresistance of tumor cells. As a strategy for compromising Ras protein activity and potentially enhancing the radiosensitivity of tumor cells, we have investigated the application of the AV1Y28 adenovirus, which expresses a single-chain antibody fragment directed against p21 Ras proteins. The ability o...

OBJECTIVE To identify the prognostic significance of certain clinical, cellular and immunologic markers in resectable non-small cell lung cancer (NSCLC). DESIGN A cohort of patients with resectable NSCLC was prospectively followed up for 8 years (100% follow-up). SETTING A university hospital in a large Canadian city. PATIENTS One hundred and thirteen consecutive patients who underwent su...

the presence of tribolium castaneum (herbst) in a stored food results in contamination and substantial economic damage due to loss of the products and a decrease in nutritional value. this study was conducted to determine the pesticidal and pest repellent activities of roots of laportea crenulata gaud. against t. castaneum. pesticidal activity was determined using surface film method, whereas, ...