The current status of antenatal genetic diagnosis is reviewed and the limitations of present techniques are discussed. It is suggested that multidisciplinary clinics are the most efficient means of providing this aspect of health care. Advances in cell culture techniques, in ultrasonography and in fetoscopy will extend the services available, and the impact of this will be felt by the community...

Perfusion culture optimization in multiple noninstrumented small-scale flasks allows reduced expense and time associated with process development. These cultures normally use a different process mode because at small scales it is not practical to retain the cells for medium perfusion. In this work, the kinetics of growth, nutrient consumption, metabolite, and product formation were compared in ...

Background The increasing demand for biopharmaceuticals produced in mammalian cells has lead industries to enhance bioprocess volumetric productivity through different strategies. Among them, media development is of major interest [1]. According to the increasing constraints regarding the use of animal derived components on industrial bioprocesses but also the drawbacks of its depletion from ce...

SUMMARY Virus-like particles were recently described in an ependymoblastoma of CS7BL/J mice. For characterization of these particles, the tumor was explanted into cell culture. This study reports the growth of these cells in culture and the persistence of virus-like particles in the cells for up to 5 months in culture. The particles correspond to intracyto plasmic A particles and extracellular ...

The maintenance of high standards is fundamental to all good scientific practice, and is essential for maximising the reproducibility, reliability, credibility, acceptance and proper application of any results produced. The aim of this Guidance on Good Cell Culture Practice (GCCP) is to promote the maintenance of these standards and to reduce uncertainty in the development and application of an...

Raw materials are a critical part of any cell culture medium; therefore, it is of utmost importance to understand and characterize them for high-quality product. The raw material characterization (RMC) program at SAFC focuses on individual screening of raw materials both analytically and biologically. The goal of the program is to develop the best-in-class knowledge base of the raw materials us...

A fructose containing cell culture medium has the advantage of low lactate production and a small pH change, leading to cell and product stability. But, not all cell lines grow well in the medium, and the fructose transporter, GLUT5, is related to it [1]. Thus, we developed an efficient production system of recombinant proteins by metabolic control and co-expression with GLUT5 in a fructose-bas...

The work presented here demonstrates the utility of a two-step algorithm for environmental poliovirus surveillance based on: preselection of sewage samples tested for the presence of enteroviral genetic material-RT-PCR assay and detection of infectious viruses by cell culture technique (L20B for polioviruses and RD for polio and other non-polio enteroviruses). RD and L20B cell lines were tested...

Fed-batch culture strategy is often used for increasing production of heterologous recombinant proteins in Escherichia coli. This study was initiated to investigate the effects of dissolved oxygen concentration (DOC), complex nitrogen sources and pH control agents on cell growth and intracellular expression of streptokinase (SK) in recombinant E. coli BL21(DE3). Increase in DOC set point from 3...

The original report of Hiatt (1989) initiated a wave of excitement at the realization that a complex mammalian protein such as immunoglobulin could be assembled within a plant cell. The general reaction was one of amazement, but interest in exploiting the possibilities arising from the discovery, for example to make antibodies of therapeutic value, has taken a considerable time to develop. In t...