Background During cultivation cells undergo a tremendous change in their metabolism when shifting from one state to another or when parameters are changed. To understand the changes in intracellular metabolite concentrations and their impact on cell performance we used a systematic approach. By employing the chemostat mode at different steady state conditions we investigated the alterations of ...

0x1.~ limited success has been obtained in tieveloping strains of human cells that can be cultivateti for long periods of time in uitro and that still preserve the diploid chromosomal configuration [41, 47, 48, 58, 591. Indeed, heteroploidy may be a necessary corollary or even the cause of the alteration of primary or tliploid cells in uitro to the status of a cell line. Such changes i n chromo...

Background Sodium butyrate (NaBu) is not only known to inhibit proliferation but also to increase the specific productivity in cultivation of Chinese hamster ovary (CHO) cells [1] – the most commonly used mammalian cell line for pharmaceutical protein production [2]. So far, little is known about the underlying mechanisms and genes that are affected by butyrate treatment. Besides the proteomic ...

Introduction Conventional biopharmaceutical manufacturing is characterized by validated process steps and extensive lab testing procedures. The FDA PAT-Guidance recommends the use of potential for improving development, manufacturing, and quality assurance through innovation in product and process development, process analysis and process control. Measurement of glucose, as a major nutrient dur...

BACKGROUND Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen depletion, lack of pH control and the necessity to use low induction cell densities. In this article we describe the impact of a nove...

In this research the microorganism was initially isolated and selected after evaluation based on COD reduction of cheese whey and biomass production. The selected microorganism was identified as Trichosporon sp. The cultivation conditions of the microorganism were optimized under batch: temperature 30˚C initial pH = 6 aeration speed = 2 ν.ν.m and agitation rate: 800 rpm. Under these conditions,...

Glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 PDO). Here we report a newly isolated bacterial strain BA11 from soil, capable of fermenting glycerol to 1,3 PDO, and has been identified to be a strain of Klebsiella pneumoniae. Strain BA11 was fast growing showing peak 1,3 PDO production in 6 h of cultivation with productivity of 1.2 g/...

S U M M A R Y Stable single-domain (SD) magnetite formed intracellularly by magnetotactic bacteria is of fundamental interest in sedimentary and environmental magnetism. In this study, we studied the time course of magnetosome growth and magnetosome chain formation (0–96 hr) in Magnetospirillum magneticum AMB-1 by transmission electron microscopy (TEM) observation and rock magnetism. The initia...

The behavior of embryonic murine lung cells on a basement membrane extract (Matrigel) was investigated. Single cell suspensions generated by trypsinization of lungs removed from day 12 embryos were plated on Matrigel and cultured for up to one week. The basement membrane extract was used as a gel, and as a wet or dried film. In all of these instances, organotypic arrangement of the embryonic lu...