Heparin is widely used as the anticoagulant of choice for cardiopulmonary bypass. However, some patients exposed to heparin therapies develop heparin-induced thrombocytopenia (HIT). Severe complications of HIT-induced thrombosis may lead to end-organ dysfunction and death. Bivalirudin, a hirudin analog, is an alternative anticoagulant that may be used in the patient with HIT without inducing th...

The literature reviewed discussed the varying practices of anticoagulation measurement in those open heart patients receiving aprotinin. All references have reported an increase in celite ACTs (C-ACTs) in heparinized patients who were treated with aprotinin. Two authors attributed this effect to aprotinin's ability to enhance heparin's anticoagulation and therefore permit a decrease in the hepa...

Mitochondrial porin forms channels in the outer mitochondrial membrane which allows the passage of molecules with diameters up to -20 A [l-6]. We have described the purification of this protein [7] from purified outer membranes by differential extraction with detergents and anionic exchange chromatography. The purified porin displayed an app. Mr of 31 000 as judged by polyacrylamide gel electro...

An analyt~cal method has been developed which can be used for routine detect1011 and estiinatlon of oligosacchar~des in cane sugar products. Levels as low as 0.01% can be detected and oligosacchaiides have beell found in all m~l l and refinery products. The method IS based on giadient elutlon wlth aqueous ethanol, usiilg a carbon-celite column. Samples of about zoo mg glve good results for most...

In order to increase the sensitivity of the assay for ceramide: UDPGlc glucosyltransferase, the enzyme that makes glucocerebroside, we synthesized a variety of ceramide homologues that might be better substrates than the naturally occurring ceramides. N-Octanoyl sphingosine proved to be the best lipid tested in liver and brain. It could be added to the tissue homogenate in the dry form, as a th...

BACKGROUND A fermented beverage of plant extracts was prepared from about fifty kinds of vegetables and fruits. Natural fermentation was carried out mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp. and Pichia spp.). We have previously examined the preparation of novel four trisaccharides from the beverage: O-beta-D-fructopyranosyl-(2->6)-O-beta-D-glucopyranosy...

The N-terminal structure of adult Japanese monkey (macaca fuscata fuscata) hemoglobin and the N-terminal amino acid analyses of the tryptic peptides from this hemoglobin were performed. Hemoglobin, after removal of heme, was dinitrophenylated. The DNP-globin was hydrolyzed with HCl, and from the hydrolysate DNP-derivatives were extracted with ether or ethyl acetate. They were isolated and ident...

1. A modification of the RPC 1 system of A.D. Kelmers, G.D. Novelli & M.P. Stulberg (1965) (J. Biol. Chem. 240, 3979-3983) is described in which the support medium is a Celite of narrow range particle size treated with dichlorodimethylsilane. 2. By using this system an apparently pure preparation of tRNA Cys was isolated from baker's yeast tRNA. 3. This preparation accepted at least 60% of the ...

BACKGROUND Allopregnanolone (3α-hydroxy-5α-pregnan-20-one) is a neurosteroid which has an inhibitory function through interaction with the GABAA receptor. This progesterone metabolite has strong sedative and anxiolytic properties, and low endogenous levels have been associated with depressed mood. This study aimed to investigate whether the very high serum allopregnanolone levels in late pregna...

Heparin has been conventionally used as an anticoagulant for medical and surgical indications. Because factor Xa is an essential component of the prothrombinase complex and leads to the generation of thrombin, its inhibition has become a focus of newer antithrombotic drug development. The in vitro anticoagulant profile of DX-9065a, a synthetic direct factor Xa inhibitor, was studied using activ...