collagens are the most abundant proteins in the human body. their main function is to provide structural and mechanical support for the tissues, but they are also involved in a number of other biological functions including cell attachment, migration and differentiation. collagens and gelatins are widely used in pharmaceutical and medical applications. every year, more than 50,000 tons of colla...

Ueli Gubler Department of Inflammation and Autoimmune Diseases Hoffmann-La Roche Inc. 340 Kingsland Street Nutley, NJ 07110-1199 After finishing graduate school In Switzerland in December 1979, I moved to the US to become a postdoctoral fellow at the Roche Institute of Molecular Biology under the direction of Pat Gage. The next year, my project required the generation of a cDNA library. With a ...

We constructed a "cDNA bank" of human colorectal cancer and surrounding normal tissues with our unique mRNA assay system. Total nucleic acids extracted from patients' tissues were applied to 96-well plates, where poly(dT) sequences of oligonucleotides were immobilized. After hybridization, the cDNA was reverse-transcribed on the plate with the captured mRNA as a template, followed by synthesis ...

aflatoxins are toxic compounds which are produced by aspergillus flavus and a. parasiticus. these toxins can be transmitted into the animal body via contaminated feed. when these toxins enter the animal body, they can penetrate into different tissues. one of the most important tissues which can be influenced by aflatoxin is the mammary gland. milk protein is one the most important factors in de...

background: as the third most frequent cause of cancer death, breast cancer is a common disease worldwide. most of the patients are being diagnosed in the stage that conventional treatments are not effective, and invasion and metastases lead to death. therefore, identification of novel molecular markers to improve early diagnosis, prognosis and treatment of the breast cancer is a necessity. zin...

sue samples using Proteinase K digestion method. A 5 ug of the viral RNA was used for the synthesis of cDNA using RevertAid TM First Strand cDNA Synthesis Kit (MBI, Fermentas). The VP2 hypervariable region of the IBDV was amplified using specific primers. A single band of PCR product of the expected size was visualized on 1.5% agarose gel after ethidium bromide staining. Results indicated that ...