Plus/minus screening of the rabbit corneal cDNA library was performed using corneal and iris RNA as probes. Thirteen clones were isolated: three ferritin H-chains, a NADH-ubiquinone oxidoreductase B22 subunit, an alpha 1 type VIII collagen, a 25 KDa FKBP-506 binding protein (FKBP25), a thrombospondin 2, and six unknown clones. Although proteins translocated from these isolated mRNA are not corn...

The sequence presented was obtained by screening a cDNA library constructed in lambda gtlO from human mammary carcinoma with probes derived from cDNA of the translationally controlled, growth-related mouse tumor protein p23 (1,2). It has 86% similarity to the mouse cDNA sequence and encodes a protein with 96% similarity to the mouse tumor protein. The 3-primed noncoding region of the appropriat...

This paper analyzes the actuality and requirements of the integration of different-structure and distributed education resources. At present, the service mode of the education resource library system based on the network is changing from Client/Server mode to Browser/Server mode which generally uses ordinary WEB Browser as the user interface. However, from the use effect of recent years, the qu...

Transcription factors (TFs) have been difficult to identify by bioinformatics, due to the heterogenous nature of the domains they are composed of. Therefore, we have developed a simple and generally applicable screening system for the identification of transcriptional activators, based on the presence of a functional transactivation domain (TAD). The system utilizes a retroviral vector to expre...

To complement cDNA libraries from the human eye at early gestation and to discover candidate genes associated with early ocular development, we used freshly dissected human eyeballs from week 9-14 of gestation to construct the early human fetal eye cDNA library. A total of 15,809 clones were isolated and sequenced from the unamplified and unnormalized library. We screened 11,246 good-quality ES...