Women aged 67-84 yr were randomly assigned to either resistance exercise (RE, n = 15) or control group (C, n = 14). RE group completed 10 wk of resistance training, whereas C group maintained normal activity. Blood samples were obtained from the RE group (at the same time points as for resting C) at rest, immediately after resistance exercise, and 2 h after exercise before (week 0) and after (w...

The murine T-lymphoma cell line LBRM-33 is known to require synergistic signals delivered through the antigen receptor (Ti-CD3) complex, together with interleukin 1 (IL-1), for activation of IL-2 gene expression and IL-2 production. Although 12-O-tetradecanoylphorbol-13-acetate (TPA) was capable of replacing IL-1 as an activating stimulus under certain conditions, biologic studies indicated tha...

Transgenic mice carrying and expressing the human CD3 epsilon gene incorporate the corresponding protein product into T cell receptor (TCR)/CD3 complexes on thymocyte and T cell surfaces. The chimeric antigen receptors allow normal T cell development and selection of repertoires in vivo and are able to transduce activation signals in vitro. We have exploited the ability to distinguish mouse (m)...

Expression of programmed cell death receptor ligand 1 (PD-L1) has been shown to be up-regulated in some gastric cancer patients and to correlate with the density of tumour infiltrating lymphocytes (TILs). However, conflicting results have been reported regarding TILs and the expression of PD-L1 as a prognostic marker for gastric cancer. We investigated the correlation of PD-L1 and TILs expressi...

In endometriosis, an aberrant immune response in the peritoneal environment is evident, but underlying mechanism remains unclear. recent years, some emerging data support important role for changes intracellular metabolic pathways of cells controlling their function. this study, we aim to investigate phenotypic and immunometabolism profiling fluid endometriosis patients. By flow cytometry, our ...

キムリア製造には，原料細胞として必要十分なCD3＋リンパ球を採取する必要がある．本研究では，Tisagenlecleucel治療を目的としたリンパ球採取を行った30例（小児5例・成人25例）を後方視的に解析し，血球自動分析装置で測定した採取途中産物中リンパ球数（CBC-Ly）と採取前末梢血CD3＋比率から最終産物のCD3＋リンパ球数が予測可能であるか検討した．

Immunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on market ferrite coated with a silicon shell. Based new type of magnetic material, graphene iron nitride particle (G@FeN-MP), which we previously reported, prepared novel IMB, oxide immune bead (GO@FeN-IMBs), explored its feasibility sorting. First, surface G@FeN-MP was oxid...

Background: Lymphocyte subsets enumeration is considered prominent in the management of primary and acquired immunodeficiency disorders. Because of local variations due to race, age, gender, and environmental conditions on lymphocyte subsets, and to improve the accuracy of interpretation of laboratory findings, reference intervals must be determined in every population. Objective: To establish ...

Infertile marriage is a complex socio-economic problem; the frequency of infertility in Ukraine 17-20%, which prompts further study its mechanisms, including immune ones, order to develop appropriate methods correction. Purpose - characterize phenotypic features T-cells and their subpopulation T-helpers (CD3+CD4+) with expression intracellular cytokines women history at different stages pregnan...