A Cd2+-selective vibrating microelectrode was constructed using a neutral carrier-based Cd ionophore to investigate ion-transport processes along the roots of wheat (Triticum aestivum L.) and two species of Thlaspi, one a Zn/Cd hyperaccumulator and the other a related nonaccumulator. In simple Cd(NO3)2 solutions, the electrode exhibited a Nernstian response in solutions with Cd2+ activities as ...

background: diffuse large b-cell lymphoma (dlbcl) is an aggressive malignancy of mature b lymphocytes. it is known as a heterogeneous disease with variable therapeutic responses and alternative therapies. morphological and immunophenotypical evaluation of the biopsy specimens can help diagnose dlbcl. methods: in the current study, 44 patients were chosen from shaheed sadoghi hospital (2010–2013...

We have identified a rare ( approximately 0.05-0.1%) population of cells (Thy-1(hi)CD16(+)CD44(hi)CD2(-)TCRalphabeta(-)B220(-)M ac-1(-)NK1. 1(-)) in the adult mouse bone marrow that generates CD4(+) and CD8(+) TCRalphabeta(+) T cells after tissue culture for 48 hr in the presence of Ly5 congenic marrow cells. The essential stages in the maturation of the progenitors were determined; the stages ...

Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease with deep-seated lesions in the apocrine gland areas of body. Recruitment and activation innate adaptive immune cells around hair follicles drive initiation progression HS; however, nature cell populations underlying mechanisms remain unclear. scRNAseq revealed that NK CD4 T were major lymphocyte HS. Confocal microscopy showed...

The present study synthesizes a novel adsorbent by coating Fe3O4 magnetic nanoparticles with amino functionalized mesoporous silica. The FTIR spectrums indicate that silica has been successfully coated on the surface of Fe3O4 and 3-aminopropyl tri methoxysilane compound have been grafted to the surface of silica-coated Fe3O4. The XRD analysis shows the presence of magnetite phase with cubic spi...

We investigated the potential utility of a recombinant E. coli that expresses the human metallothionein II gene as a fusion protein with beta-galactosidase as a heavy metal biosorbent. E. coli cells expressing the metallothionein fusion demonstrated enhanced binding of Cd2+ compared to cells that lack the metallothionein. It was shown that the metallothionein fusion was capable of efficiently r...

Metallothionein (MT) is a metal-binding protein rapidly accreted in many tissues in response to trace elements or hormones. To gain an understanding of the regulation of MT accretion, rates of MT synthesis and degradation were determined by using a decay-kinetics technique. A chicken macrophage-cell line (HD11) that rapidly accretes incremental amounts of MT when stimulated with increasing conc...

Poly(HEMA) cryogel disks were synthesized by free radical polymerization of 2-hydroxyethylmethacrylate (HEMA), and then Cibacron Blue F3GA (CB), Reactive Green 19 (RG) and Congo Red (CR) were immobilized as dye ligands. Disks were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and swelling degree, porosity calculations. Cd2+ adsorption experi...

Tomato cell suspensions have been selected for sustained growth on normally lethal concentrations of CdCl2. In cadmium-resistant (CdR) cells, Cd2+ is found complexed with non-protein, cysteine-rich polypeptides which accumulate in high amounts when cells are grown in the presence of Cd2+. Sequence and linkage analysis of these peptides by triple quadrupole mass spectrometry establishes structur...