نتایج جستجو برای: ccrf
تعداد نتایج: 457 فیلتر نتایج به سال:
cently established and well characterized both morphobogi cabbyand biochemically (11). Due to their unique growth ct@'@cteristics and increased sensitivity to a variety of chem icab .gents (6), some of the techniques normally used for synchronization of nonlymphoid cells are not directly appli cable to these cell lines. The recent availability of flow microfluorometry for rapid determination of...
In the past decade, several dedicated tools have been developed to help natural resources professionals integrate climate science into their planning and implementation; however, it is unclear how often these lead on-the-ground adaptation. Here, we describe a training approach that managers effectively plan execute intentional, climate-informed actions. This was through Climate Change Response ...
Starting with an established line of human leukemic lympho blasts (CCRF-CEM), we have developed sublines with varying degrees of resistance to vinblastine by growth in the presence of sublethal concentrations of drug. The resistance of these sublines to vinblastine mangesfrom 10.6-fold to more than 2000-fold when compared with the sensitive parent cells. These vinblastine-resistant cells tend t...
A subset of peripheral blood T lymphocytes coexpressing CD3 and IgG Fc receptors (FcR) (CD16/Leu-11 antigen) have been identified, isolated, and functionally characterized. The CD3+, CD16+ cells were established in short-term culture using growth medium containing interleukin 2 (IL-2). Both the freshly isolated cells and the cultured cell line stably expressed the CD3+, CD16+ phenotype. Further...
9-Beta-D-Arabinosylguanine (ara-G) is a recently introduced and effective treatment for T-cell acute lymphoblastic leukemia, but how ara-G and ara-G triphosphate (ara-GTP) kill cells is not known. We hypothesized that, in cycling T-lymphoblastoid cells, ara-G may act directly by incorporation into DNA, which may lead to apoptosis. Hence, blocking the incorporation of ara-G monophosphate (ara-GM...
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