Mammalian seminal plasma contains many distinct components which are important for spermatozoal function and survival. Recent studies clearly indicated that gossypol has pronounced hazardous effects on seminal characteristics and seminal plasma constituents in male rabbits, which may lead to deleterious productive and reproductive performance. We examined the possible deleterious effects of two...

Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In th...

The present research was undertaken to determine the role of seminal plasma in heat stress (HS) infertility. Males were exposed to HS at 32 C or maintained at 21 C as controls. Centrifugation and reconstitution of semen samples created four final treatments: control sperm + control plasma (CsCp), control sperm + HS plasma (CsHp), HS sperm + HS plasma (HsHp), and HS sperm + control plasma (HsCp)...

Boar spermadhesin AWN-1 is a sperm surface-associated 14.7-kDa lectin and a major protein of porcine seminal plasma. AWN-1 binds to beta-galactosides and to porcine zona pellucida glycoproteins, suggesting that this protein might play a role in the primary binding of spermatozoa to the egg's external glycoprotein matrix. We have produced a collection of murine monoclonal antibodies against puri...

The cervix is central to the female genital tract immune response to pathogens and foreign male Ags introduced at coitus. Seminal fluid profoundly influences cervical immune function, inducing proinflammatory cytokine synthesis and leukocyte recruitment. In this study, human Ect1 cervical epithelial cells and primary cervical cells were used to investigate agents in human seminal plasma that in...

and inactive fractions, with RF values of 0.15 and 0.34. Sliced gels eluted into NaHPOJ KH2P04 buffer, pH7.0, over 24h at 4°C showed the latter band to be inactive and the former to contain fumarase activity. Addition of the inactive protein to the active enzyme caused complete inhibition of activity. Dialysis of the initial pooled inactive protein over 36h resulted in high fumarase activity in...

Background: Oxidative stress and decreased antioxidant levels have been projected as a potential factors involved in the pathophysiology of diverse male infertility types, including asthenospermia. The generation of reactive oxygen species (ROS) in the male reproductive tract has become an actual concern because of their probable noxious effects, at high levels, on physical properties of sperm ...

The in sacco protein and protein-free dry matter rumen degradability of three feeds, concentrate, alfalfa hay and maize silage, was determined simultaneously in three animal species: four Mediterranean buffalo (Bubalus bubalis) bulls, four Friesian bulls and four Delle Langhe rams, which received four diets differing in forage:concentrate ratio (1 5 87.5:12.5; 0.75 2 5 75.0:25.0; 3 5 62.5:37.5;...

Gender selected or sexed semen has been commercially available to the dairy industry for almost a decade. However, sexed semen from beef bulls has only recently become commercially available. The availability of sexed semen from beef bulls along with concerns about success of the technology at the ranch level has limited the use of sexed semen in purebred and commercial beef operations. Recent ...