Chlorodifluoromethane (HCFC-22) is one of the air pollutants that destroy ozone layer and warm earth. In order to degrade HCFC-22, catalytic hydrolysis method currently most effective environmental technology. Herein, we prepare solid acid MoO3/ZrO2 base MgO/ZrO2 catalysts for degradation with a particular focus on revealing effects catalyst preparation methods, calcination temperatures, temper...

<span>Banana stem midrib has potential as glucose source through catalytic hydrolysis assisted by ultrasonic (sono-hydrolysis). Besides, natural sand-based silica is raw material for zeolite synthesis. The purpose of the study was to do two things: i) characterize HY-zeolite made from and ii) determine whether or not effective sono-hydrolysis banana midrib. There a total four processes th...

in this study fullerene functionalized using oleum (h2so4·so3), followed by the hydrolysis of the intermediate cyclosulfated fullerene as well as  an oxidizing agent was employed to functionalize the fullerenes. ruthenium was then added by the impregnation method or deposited on the functionalized fullerene. subsequent to this step, response surface methodology (rsm) was used to study the cumul...

Resorufin acetate is a very good substrate for sheep liver cytosolic aldehyde dehydrogenase, both from the point of view of practical spectrophotometry and in terms of information provided about the nature of the catalysis shown by this enzyme. p-Nitrophenyl (PNP) acetate competes against resorufin acetate for the enzyme's active site (although relatively weakly as the latter substrate has the ...

Escherichia coli alkaline phosphatase (AP) is a proficient phosphomonoesterase with two Zn(2+) ions in its active site. Sequence homology suggests a distant evolutionary relationship between AP and alkaline phosphodiesterase/nucleotide pyrophosphatase, with conservation of the catalytic metal ions. Furthermore, many other phosphodiesterases, although not evolutionarily related, have a similar a...

Pilocarpine has been widely used in ophthalmic preparations for the treatment of glaucoma and in oral preparations for the treatment of radiation-induced xerostomia and Sjögren syndrome. The major metabolic pathways of pilocarpine in human are hydrolysis and hydroxylation. It was found that CYP2A6 is responsible for the 3-hydroxylation, but the enzymes responsible for the hydrolysis have not be...

The roles of ATP hydrolysis in electron-transfer (ET) reactions of the nitrogenase catalytic cycle remain obscure. Here, we present a new structure of a nitrogenase complex crystallized with MgADP and MgAMPPCP, an ATP analogue. In this structure the two nucleotides are bound asymmetrically by the Fe-protein subunits connected to the two different MoFe-protein subunits. This binding mode suggest...

Response surface analysis was used to determine optimum conditions [2% (w/v) chitin, 57.5 degrees C, 38 min] for microwave irradiation of chitin to improve its enzymatic hydrolysis. V(max)/K(m) of cabbage chitinase toward untreated and microwave-irradiated chitin was found to be 21.1 and 31.7 nmol h(-1) mg(-2) mL, respectively. Similar improvement was observed in the case of pectinase in its un...