KRASTANOV, A., A. GOVINDARAJAN and D. DANIEL, 2008. Studies on lipase fermentation using Candida cylindracea NRRL Y-17506 in a stired tank bioreactor. Bulg. J. Agric. Sci., 14: 290-299 Lipase is an enzyme of considerable commercial and industrial importance. The major applications of lipase are in the fat and oleochemical, food and dairy, textile, detergent, cosmetics, tea processing, pulp and ...

Background Candida rugosa Lipase (CRL) shows a very low alkaline stability that comprises its immobilization on glyoxyl-agarose, which requires pH above 10. In this way, an adaptation from the original method was used; an enzyme solution at pH 7 was slowly added at a suspension of glyoxyl-agarose prepared in bicarbonate buffer, pH 10. This change of protocol was enough for allowing the preparat...

The application of the climatic chamber presented in this paper to assess storage stability immobilized lipases is a new approach characterized by potential unifying study conditions biocatalysts created various laboratories. data achieved from storing chambers may be crucial for chemical and pharmaceutical industry. Our describes developed protocols immobilization via interfacial activation li...

Caspofungin is being used increasingly as therapy for invasive candidiasis. Prospective sentinel surveillance for emergence of in vitro resistance to caspofungin among invasive Candida spp. isolates is indicated. We determined the in vitro activity of caspofungin against 8,197 invasive (bloodstream or sterile-site) unique patient isolates of Candida collected from 91 medical centers worldwide f...

This study reports the immobilization of Candida Rugosa lipase (CRL) onto nanocellulose (NC) extracted from almond shells using p-toluenesulfonic acid (PTSA) and sulfuric (ASS) with sugar-based natural deep eutectic solvent (NADES1a) as a biocatalyst system. The properties both immobilized lipases were studied compared to free enzyme counterpart. Under optimized conditions (2 h, 40 ºC pH 7.0), ...

Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl)adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immo...

OBJECTIVE Amphotericin B failure is frequently seen in patients with candidaemia caused by Candida rugosa. We evaluated amphotericin B, fluconazole, posaconazole and voriconazole as alternative treatments against infection in mice with two isolates of C. rugosa. METHODS Neutropenic mice were inoculated intravenously with C. rugosa. Amphotericin B, fluconazole, posaconazole and voriconazole we...