to explore the potential for in vitro rapid regeneration of fagonia indica burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on ms medium which supplemented with 1.0-5.0 µm 2,4-d. callus was initiated from cotyledon explant on ms medium supplemented in all concentrations of 2,4-d investigated. 2,4-d was more effective than iaa and naa for cal...

An efficient regeneration system was developed for Kigelia pinnata L., a multipurpose tree belonging to the family Bignoniaceae. The nodal segments were cultured in vitro, and the optimum concentrations of plant growth regulators for callus induction were determined. The friable organogenic calli were derived from the basal cut end of the nodal segments. The highest yield of morphogenic callus ...

Plants develop unorganized cell masses like callus and tumors in response to various biotic and abiotic stimuli. Since the historical discovery that the combination of two growth-promoting hormones, auxin and cytokinin, induces callus from plant explants in vitro, this experimental system has been used extensively in both basic research and horticultural applications. The molecular basis of cal...

Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg ...

A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% su...

The morphogenic potential and free-radical scavenging activity of the medicinal plant, Silybum marianum L. (milk thistle) were investigated. Direct root regeneration and indirect organogenesis and preliminary phytochemical, antibacterial studies of its callus. From leaf explants the highly significance result was found on the NAA at 2 mg/l and KIN at 0.2 mg/l for the direct root induction and i...

A highly efficient protocol for in vitro propagation of Rauwolfia serpentina L. was developed that consisted of callus induction, multiple shoot regeneration and rooting stages. Effect of different plant growth regulators, exclusively or in combination on callus induction, multiple shoot formation and root induction, were observed. Maximum callusing (93.65%) were obtained in leaf and stem expla...

Abstract Polyploidy induction could increase shallot bulb-size to raise consumer preference and local productivity. The research aimed obtain an effective method of polyploidy on callus onion ( Allium cepa ) var. Bima Brebes. experiment was consisted two experimental steps, which were polyploid the callus. A 1×1 cm treated by drops oryzalin with concentrations 0, 25, 50, 75, 100, 120 ?M. ploidy...

The present investigation was carried out to develop an efficient in vitro regeneration protocol for strawberry plant using leaf explants. highest percentage of callus induction obtained on MS medium supplemented with 2.0 mg/l BAP and 0.5mg/l IBA. numbers shoots were regenerated from the 1.5mg/l In case root induction, 1 IBA found be most effective. Following development roots, plantlets succes...