The simple and easy performed automated method for the IR determination of petroleum products (PP) in water using extraction-chromatographic cartridges has been developed. The method assumes two stages: on-site extraction of PP during a sampling by using extraction-chromatographic cartridges and subsequent determination of the extracted PP using sequential injection analysis (SIA) with IR detec...

Horseradish peroxidase was incorporated in a kieselguhr membrane. The electron-transfer process of the enzyme was examined by cyclic voltammetry. It was observed that the electron-transfer reactivity of horseradish peroxidase was greatly enhanced, and that direct electrochemistry was accordingly feasible. Using the merits of the direct electron-transfer reactivity of horseradish peroxidase and ...

Development of an immunoassay for clenbuterol (CLB) detection required an anti-CLB antibody as an important bioreceptor. In this study, we report our work on production and purification of a rabbit-derived polyclonal anti-CLB antibody. The antibody was then purified by nProtein A Sepharose affinity column and the antibody purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electro...

Polarimeters are useful instruments that measure concentrations of optically active substances in a given solution. The conventional polarimetric principle consists of measuring the rotation angle of linearly polarized light. Here, we present a novel polarimeter based on the study of interference patterns. A Mach-Zehnder interferometer with linearly polarized light at the input is used. One bea...

Nifedipine and NifedipineD6, (IS) were extracted from the plasma by precipitation method and then separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min. The analytes were detected in API 4000 Mass spectrometer in the positive atmospheric pressure chemical Ionization (APCI) mode with multiple reactions monitoring ...

Upon dispersant-assisted exfoliation, pristine carbon nanotubes (CNTs) are divided between the supernatant and precipitate, which makes the determination of dispersant concentration a challenging task. We have developed a thermogravimetric-spectroscopy-based approach to accurately determine the dispersant-assisted CNT (or nanoparticles, in general) concentration in dispersion. A thermogravimetr...

A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD⁺ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo refere...

An alternative formulation of the Langley plot relating observed solar irradiance, extraterrestrial solar irradiance, and air mass has been suggested to potentially improve radiometer calibration accuracy. In this study, results from the traditional and alternative plotting methods are compared using both simulated and measured data. The simulations indicate that their relative accuracies depen...

Mycotoxins food contamination represents a serious risk for consumers health. They are 12 secondary metabolites of fungi that can be present in a wide range of foodstuffs. Ochratoxin A 13 (OTA) is one of the most toxic compound and it is classified as a possible carcinogenic molecule. 14 The harmful effects of OTA on human and animal health lead to a big boost to develop and 15 optimize highly ...