Dengue fever is an infectious tropical disease which is caused by the dengue virus. Dengue infection is one of the most significant mosquito-transmitted infections which is common in >100 tropical and subtropical countries. Dengue fever is becoming a serious health risk these days and it shortly needs treatment against the increasing problems around the globe and the existence of resisting muta...

Non-homologous end joining (NHEJ) is a major DNA double strand breaks (DSBs) repair pathway that maintains genome integrity. However, this pathway may reduce radiotherapy efficacy by repairing DSBs on cancer cells. This research reported a computer-aided drug design (CADD) method to identify novel inhibitors from traditional Chinese medicine (TCM) that disrupt NHEJ. We aim to inhibit Ku86, the ...

Exome sequencing is becoming a standard tool for gene mapping of genetic diseases. Given the vast amount of data generated by Next Generation Sequencing techniques, identification of disease causal variants is like finding a needle in a haystack. The impact assessment and the prioritization of potential pathogenic variants are expected to reduce work in biological validation, which is long and ...

Lipoxygenases (LOXs) are non-heme iron containing dioxygenases involved in the oxygenation of polyunsaturated fatty acids (PUFAs) such as arachidonic acid (AA). Depending on the position of insertion of oxygen, LOXs are classified into 5-, 8-, 9-, 12- and 15-LOX. Among these, 5-LOX is the most predominant isoform associated with the formation of 5-hydroperoxyeicosatetraenoic acid (5- HpETE), th...

The in silico prediction of the functional consequences of mutations is an important goal of human pathogenetics. However, bioinformatic tools that classify mutations according to their functionality employ different algorithms so that predictions may vary markedly between tools. We therefore integrated nine popular prediction tools (PolyPhen-2, SNPs&GO, MutPred, SIFT, MutationTaster2, Mutation...

Tumour necrosis factor is a polypeptide cytokine involved in inflammation and the acute phase response. TNF-alpha is present in larger quantities in persons with rheumatoid arthritis. Direct inhibition of TNF-α by the commercial biological agents has produced significant advances in rheumatoid arthritis treatment and validated the extra-cellular inhibition of this proinflammatory cytokine as an...

Precast load-bearing shear wall panels are used extensively for high-rise construction because of the ease and speed of assembly, and the high quality of the precast panels. The connections between panels are extremely important since they affect both the speed of erection and the overall integrity of the structure. This paper presents the results of a research program conducted to investigate ...

Despite the low rate of sewer service coverage in developing countries, especially small towns and rural areas, decentralized wastewater treatment systems (DEWATS) are alternatives to ensure public health. Nonetheless, understanding physical or functional aspects these system constructions remains difficult when there is no bibliography, a teaching professional, even prototype that allows techn...

Computer-Aided Drug Design (CADD) approaches, such as those employing quantitative structure-activity relationship (QSAR) methods, are known for their ability to uncover novel data from large databases. These approaches can help alleviate the lack of biological and chemical data, but some predictions do not generate sufficient positive information be useful screenings. QSAR models often employe...