There is now considerable evidence that spontaneous leukemias in mice are caused by C-type RNA viruses. These viruses appear to be capable of existing in the animal in an unexpressed form for many generations. In inbred mouse strains such as C58 and AKR, in which virus can be detected early in life, leukemia develops at high frequency in young animals (1, 2). In other strains such as BALB/c or ...

Bacillus subtilis strain IH7 was isolated from the rhizosphere of Tunisian healthy plants and selected for its antimicrobial activities against bacteria and fungi. This strain produced a novel antimicrobial peptide (bacteriocin) called Bac IH7. The pure bacteriocin was obtained after precipitation by ammonium sulphate, chromatography on Sephadex G-25 and C18 reverse-phase HPLC. The SDS-PAGE ana...

A procedure is presented, that has allowed the rapid assignment of transposon Tn1 and Tn7 insertion sites in the large (130 Md) nopaline Ti-plasmid pTiC58, to specific restriction enzyme fragments. Total bacterial DNA is isolated from Agrobacterium tumefaciens strain C58 mutants that carry a transposon in their Ti-plasmid, and digested with an appropriate restriction endonuclease. The fragments...

A new cell surface antigenic system of the mouse, designated G(RADA1), is described. The antigen is defined by cytotoxic tests with the A strain X-ray-induced leukemia RADA1 and naturally occurring antibody from random-bred Swiss mice and can be distinguished from all other serologically detected cell surface antigens of the mouse. Absorption tests indicate that G(RADA1) is present in the norma...

for lung tissue (29). Subsequently, it has been shown to produce tumors in a variety of tissues of the mouse (37). Hemorrhagic lesions (6, 34, 37) and hemangiomas (20, 22, 32) in the livers of mice have been observed following the administration of unethan. Heston et al. (18) provided data obtaine4 from unethan-tneated C3H mice that permitted the addition of hepatomas to the list of neoplasms i...

Non-nodulating Agrobacterium-like strains identified among root nodule isolates of common bean were labeled with gusA, a reporter gene encoding beta-glucuronidase (GUS). Bean plants were then co-inoculated with an infective Rhizobium strain and labeled transconjugants of Agrobacterium-like strains. Blue staining of nodules showed that Agrobacterium-like strains were able to colonize these symbi...

Herein, we report a two-step deglycosylation mediated by the oxidation of glycoside which is different from traditional glycoside hydrolase (GH) mechanism. Previously, we reported a novel flavin adenine dinucleotide (FAD)-dependent glycoside oxidoreductase (FAD-GO) having deglycosylation activity. Various features of the reaction of FAD-GO such as including mechanism and catalytic residue and s...

A new common cell-surface antigen associated with murine and feline C-type RNA leukemia viruses was demonstrated by the use of rabbit antiserum against feline leukemia virus and the indirect membrane immunofluorescence test. Common cell-surface antigen was found in all leukemias of all strains of mice tested, in normal lymphoid tissues of Gross-positive (high incidence of leukemia) mouse strain...