GB virus C (GBV-C), also known as hepatitis G virus, is a recently discovered flavivirus-like RNA agent with unclear pathogenic implications. To investigate whether human peripheral blood mononuclear cells (PBMC) are susceptible to in vitro GBV-C infection, we have incubated PBMC from four healthy blood donors with a human GBV-C RNA-positive serum. By means of (i) strand-specific reverse transc...

In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture mediawere collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically andincubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h...

most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. the general use of nmri outbred mice is a model for toxicology, teratology and pharmacology. nmri mouse embryo exhibit the two-cell block in vitro. the objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...

Screening of urine specimens from men for Chlamydia trachomatis infection by a commercial PCR assay (AMPLICOR C. trachomatis Test; Roche Diagnostic Systems, Inc., Branchburg, N.J.) is a sensitive and specific noninvasive diagnostic assay. Since screening of women for C. trachomatis infection with the AMPLICOR C. trachomatis Test has been limited to use with endocervical swab specimens, we condu...

Discovery of potential therapeutics against hepatitis C virus (HCV) infection has been hampered in the past decade by the inability to grow this virus in tissue culture and by the lack of robust small animal models. This situation has been improved by the recent development of a selectable HCV replicon cell culture system. For the first time, drug discovery scientists are able to screen large c...

Twenty fungal isolates associated with leaf spots of nutsedge plants (Cyperus spp.) from north of Iran (Guilan, Mazandaran, Zanjan and Golestan Provinces) are examined. According to the results of the morphological studies of grown fungi on culture media, Curvularia clavata, Curvularia lunata var. aeria, Dactylaria higginsii, and Myrothecium brachysporum are new taxa for Iran mycoflora and repo...

an amplification polymerase chain reaction (pcr) test for the direct detection of chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. during 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specimens from ...

Asexual spores (conidia) are reproductive structures that play a crucial role in fungal distribution and survival. As fungal conidia are, in most cases, etiological agents of plant diseases and fungal lung disease, their stress resistance and interaction with their hosts have drawn increasing attention. In the present study, we investigated whether environmental temperature during conidiation a...

Be more productive and connected with significant cost savings is the Holy Grail for firm’s looking for profit maximization. In this paper, we are looking at Unified Communications and Collaboration (UC&C) technology through Unified Theory of Acceptance and Use of Technology (UTAUT) to understand the likelihood of the technology acceptance within organizational culture dimension. We investigate...

INTRODUCTION The use of a nucleic acid amplification test (NAAT) as a test of cure after treatment is subject to discussion, as the presence of C. trachomatis nucleic acids after treatment may be prolonged and intermittent without presence of infectious bacteria. We used cell culture to assess if a positive RNA- or DNA-based NAAT after treatment indicates the presence of viable C. trachomatis. ...