1. Brush borders were isolated from rabbit kidney-cortex homogenates by rate-zonal centrifugation through a sucrose density gradient in a B-XIV zonal rotor, followed by differential centrifugation. 2. The method of preparation gave brush borders of high purity with a reasonable yield. The morphological appearance supported the evidence from enzymic and chemical investigations, that the brush bo...

In the companion paper (M. D. Peterson and M. S. Mooseker (1993). J. Cell Sci. 105, 445-460) we describe a method for modeling brush border assembly in the Caco-2BBe clones. In this study we have examined the molecular changes accompanying cell contact-induced brush border assembly. A subset of brush border proteins was tracked throughout brush border assembly by immunoblotting and by immunoflu...

We consider a polymer brush grafted to a surface (acting as an electrode) and bearing a charged group at its free end. Using a second distant electrode, the brush is subject to a constant electric field. Based on a coarse-grained continuum model, we calculate the average brush height and find that the brush can stretch or compress depending on the applied field and charge end-group. We further ...

The presence of polymer chains grafted or adsorbed onto a surface can dramatically alter the forces that affect interactions between surfaces. The equilibrium properties of such polymer brush systems have been studied for the past two decades, yielding general agreement between theory and experiment. Conversely, the nonequilibrium properties of polymer brushes are still under intense theoretica...

background: lidocaine is a local anesthetic and antiarrhythmic agent. there are reports on accidental and intentional cases of poisoning following injection of lidocaine while rare are the fatal cases realized after oral ingestion of lidocaine. suicidal poisoning with lidocaine pharmaceutical formulations is rare since no pharmaceutical dosage forms for oral use are available except gels and sp...

background: formalin is an aqueous solution of formaldehyde. it is a protoplasmic poison and causes coagulation necrosis and tissue fixation. case: a 19-year-old girl was brought for autopsy to the mortuary of jss medical college, mysore. conclusion: formalin is an unusual poison to be ingested for suicidal purposes due to selective availability and its strong taste and odor.

introduction: ingestion of a foreign body is a common problem among all age groups. most of the foreign bodies in the pharynx are usually lodged at the level of cricopharynx. the diagnosis is based on history, clinical, and radiological examination. most foreign-body ingestions are accidental, but there may be contributory factors such as mental disorder, alcoholism, and prison incarceration. t...

a common belief is that alkali ingestion causes severe esophageal damage and limited gastric injury due to the buffering action of acid. gastric injury has been observed in patients who ingested alkali. gastric outlet obstruction (goo) secondary to caustic ingestion occurs due to fibrosis after resolution of the acute injury and inflammation, most commonly 6 to 12 weeks after initial ingestion....

BACKGROUND High-risk human papillomavirus (HR-HPV) infection is associated with improved prognosis and a better response to treatment in patients with oropharyngeal squamous cell carcinoma (OPSCC). Brush cytology is a noninvasive method with which to collect cells from the surface of mucosal lesions. The objective of the current study was to assess the performance of OPSCC brush cytology for th...