We developed a specific antibody to the catalytic subunit (C-subunit) of cyclic AMP-dependent protein kinase and used it to localize C-subunit in cultured cells. C-subunit antigen was purified from bovine cardiac muscle and cross-linked to hemocyanin with glutaraldehyde. Immunized goat serum showed a low titer of antibody after boosting; it was enriched 100-fold by affinity chromatography on ca...

The response of the bovine ovary to single subcutaneous injections of pregnant mares’ serum (p .m .s .) and horse pituitary extract has been studied both quantitatively and qualitatively. For the former both time-response and dose-response data were obtained, for the latter time-response data only. Quantitative changes were measured by the following ovarian indices: mean follicular diameter (m ...

When immature bovine oocytes are released from their follicles and are cultured in standard maturation medium, they resume the first meiotic division. The alteration of basic maturation conditions can affect oocyte competence significantly, as reflected by the morula and blastocyst yield after in vitro fertilization. The conditions used from the beginning of maturation up to the blastocyst stag...

The hypothesis that high concentrations of IGF1 can impair embryo development was investigated in a bovine in vitro model to reflect conditions in polycystic ovary syndrome (PCOS) patients. Embryos were either cultured in the absence or presence of a physiological (100  ng/ml) or supraphysiological (1000  ng/ml) IGF1 concentration. Cell allocation, apoptosis, transcript and protein expression o...

primary ovarian carcinoid tumors of the ovary are rare and represent less than 0.1% of ovarian malignancy. one of its subtypes is the strumal carcinoid in which the thyroid tissue is seen in intimate association with carcinoid tumor. we here report a 47-year-old woman with strumal carcinoid of the right ovary presented with cessation of menstrual periods in the past 3 months and was referred du...

Background: Follicle stimulating hormone (FSH) is one of the most common and effective gonadotropin hormones used in infertility treatment. Production of FSH as a drug is affordable as long as it is done with minimal cost. FBS is the most expensive component in medium culture furthermore; it’s being controversial to cause unnecessary suffering for the unborn calf for collection their serum. In ...

Coagulation factor IX is a serine protease for which high-level expression of biologically active protein in heterologous cells is limited due to inefficient proteolytic removal of the propeptide as well as vitamin K-dependent carboxylation of multiple amino-terminal glutamic acid residues. We have overexpressed the vitamin K-dependent gamma-carboxylase cDNA and monitored its ability to improve...

BACKGROUND Polycystic ovary syndrome (PCOS) is characterized by an adverse metabolic profile. Although dietary changes are advocated, optimal nutritional management remains uncertain. Polyunsaturated fatty acids (PUFAs), particularly long-chain (LC) n-3 (omega-3) PUFAs, improve metabolic health, but their therapeutic potential in PCOS is unknown. OBJECTIVES We aimed to determine the associati...