Axon guidance molecule Slit is critical for the axon repulsion in neural tissues, which is evolutionarily conserved from planarians to humans. However, the function of Slit in the silkworm Bombyx mori was unknown. Here we showed that the structure of Bombyx mori Slit (BmSlit) was different from that in most other species in its C-terminal sequence. BmSlit was localized in the midline glial cell...

The entomogenous fungus Nomuraea rileyi reportedly secretes a proteinaceous substance inhibiting larval molt and metamorphosis in the silkworm Bombyx mori. We studied the possibility that N. rileyi controls B. mori development by inactivating hemolymph molting hormone, ecdysteroids. Incubation of ecdysone (E) and 20-hydroxyecdysone (20E) in fungal-conditioned medium resulted in their rapid modi...

Insects are important as items of aesthetic values, pests and as food. Bombyx mori L. is an economically important insect. It is an edible insect that is eaten in the tropics. The larvae of silkworm, B. mori, are popular for silk production. Silk is produced when the larvae are ready to pupate. Both the larval and the pupal stages of B. mori were analyzed for their nutrient composition, protein...

The genetic diversity and genetic structure of three Chinese silkworm species Bombyx mori L., Antheraea pernyi Guérin-Meneville and Samia cynthia ricini Donovan were comparatively assessed based on RAPD markers. At the species level, A. pernyi and B. mori showed high levels of genetic diversity, whereas S. cynthia ricini showed low level of genetic diversity. However, at the strain level, A. pe...

The domesticated silkworm, Bombyx mori Linn., a lepidopteran molecular model and an important economic insect that are emerging as an ideal molecular genetic resource for solving a broad range of biological problems. The silkworm, B. mori produces massive amount of silk proteins during the final stage of larval development. These proteins are stored in the middle silk gland and they are dischar...

CRISPR/Cas9, a bacterial adaptive immune system derived genome-editing technique, has become to be one of the most compelling topics in biotechnology. Bombyx mori is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Here we reported highly efficient and multiplex genome editing in B. mori cell line and heritable site-directed mutagenesis of B...

*Corresponding author (e-mail: [email protected]) *Present address: Field Science Education and Research Center, Kyoto University, Kitashirakawa, Oiwake, Kyoto 606-8502, Japan. **Present address: Institute of Geochemistry, The Chinese Academy of Sciences, 46 Guanshui Road, Guiyang/Guizhou 550002, China. †Present address: NPO Mori-no-za, Ina 396-0011, Japan. ‡Present address: Graduate ...

Nucleotide sequence analysis of the Bombyx mori nuclear polyhedrosis virus (BmNPV) genome revealed the existence of a gene homologous to the p35 gene of Autographa californica NPV (AcNPV), which has been shown to prevent virus-induced apoptosis. The BmNPV p35 gene showed 96.1% nucleotide and 89.6% predicted amino acid sequence identity to the AcNPV p35 gene. A mutant BmNPV (BmP35Z) lacking a fu...

In biochemical studies of insect metamorphosis, much attention has been given to the deposition and utilization of nutrient reserves, and it has been inferred that insects may carry out net conversion of fat into carbohydrate (cf. Deuel & Morehouse, 1946; Buck, 1953; Chauvin, 1956). Since vertebrate animals are not at present known to possess any pathway for the net conversion of fatty acids in...