We describe an image analysis system developed to measure the motion of white blood cells from a temporal sequence of uorescence microscopy images. A two-pass spatio-temporal segmentation system is proposed. Pixels are classied as cell and background pixels by an initial segmen-tation in the rst pass. Region labeling, correction and cell tracking are done in the second pass. After segmentation,...

objective(s): this study describes the preparation, biodistribution of 153sm-dtpa-spion after intravenous injection in rats. materials and methods: the chelator dtpa dianhydride was conjugated to spion using a small modification of the well-known cyclic anhydride method. conjugation was done at a 1: 4 (spion: ccdtpa) molar ratio. conjugation reaction was purified with magnetic assorting column ...

The transplantation of endothelial progenitor cells (EPCs) provides a novel method for the treatment of human tumors or vascular diseases. Magnetic resonance imaging (MRI) has proven to be effective in tracking transplanted stem cells by labeling the cells with superparamagnetic iron oxide (SPIO) nanoparticles. The SPIO has been used to label and track the EPCs; however, the effect of SPIO upon...

Iron oxide contrast agents have been combined with magnetic resonance imaging for cell tracking. In this review, we discuss coating properties and provide an overview of ex vivo and in vivo labeling of different cell types, including stem cells, red blood cells, and monocytes/macrophages. Furthermore, we provide examples of applications of cell tracking with iron contrast agents in stroke, mult...

Metabolic labeling techniques are powerful tools for cell labeling, tracking and proteomic analysis. However, at present, the effects of the metabolic labeling agents on cell metabolism and physiology are not known. To address this question, in this study, we analyzed the effects of cells treated with Ac4ManNAz through microarray analysis and analyses of membrane channel activity, individual bi...

The sortase-mediated labeling method, whose applications have been limited to C-terminal labeling of cell surface proteins, has been expanded for site-specific N-terminal labeling of cell surface proteins.

The tumor-induced marrow and red blood cell cytolysis as says have been used to explore the mechanism of cancer cell destruction of normal cells. Previously, we suggested that tumor-induced cytolysis was caused by tumor cell membranebound serine proteases. In this study, we have shown that concentrations of the broad-spectrum serine protease inhibitor diisopropylfluorophosphate that did not inh...

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