A method is described for determination of 8-methoxypsoralen to 0.2 mL of suction-blister fluid or 1 mL of serum from psoriatic patients being treated with this drug. The drug is extracted from the biological matrix at pH 9.0 with a mixture of dichloromethane and light petroleum ether. 5-Methoxypsoralen is used as internal standard. Separation and quantitation are performed on a "high-performan...

Six healthy male volunteers received 1 g of azthreonam each as an intravenous injection. Serial blood and cantharides-induced blister fluid samples were taken over the next 8 h. Urine samples were collected over 24 h. The concentration of azthreonam in serum was about 50 micrograms/ml at 0.5 h after injection and fell to 2 to 3 micrograms/ml at 8 h; the serum half-life was 1.93 h, and the volum...

Volume 89, Number 1 www.aafp.org/afp American Family Physician 7 A 62-year-old man with no significant medical history presented with multiple bullae on his trunk that had been present for one week. The rash was painful but not pruritic. He did not have a fever or sore throat, and had not used any new medications. Physical examination revealed multiple vesicles, flaccid bullae, and erosions wit...

Apoptosis plays a role in pemphigus IgG-dependent acantholysis; theoretically, the blockade of the caspase pathway could prevent the blistering that is caused by pemphigus autoantibodies. Using this strategy, we attempted to block the pathogenic effect of pemphigus IgG in Balb/c mice by using the caspase inhibitor Ac-DEVD-CMK. This inhibitor was administrated before the injection of pemphigus I...

Meywa Sing, a sowar in the Xlth Bengal Lancers, while preparing to go on guard on the evening of the 21st April suddenly complained of a pain commencing in his right foot and running up the inside of his leg ; he then fell down in an insensible state, and was taken to hospital; on the morning of the 22nd, he had a similar attack to the above, which was without doubt epileptic, the aura being mo...

White pine blister rust, caused by the fungus Cronartium ribicola J. C. Fisch., is a virulent mortality agent of five-needle pines like western white (Pinus monticola D. Don.). For decades, lo...

Three major families in the order Coleoptera have been found to produce vesicant toxins, Meloidae (true blister beetles) and Oedemeridae (false blister beetles) produce cantharidin and Staphylinidae (rove beetles) produce paederin. Some of the most important distribution, biological and ecological aspects of these beetle families had been studied because of their products cause human dermatitis...

Eight healthy male volunteers received 1 g of either ceftazidime or cefamandole as an intravenous infection. Serial blood samples were taken over the next 8 h. Urine samples were collected over 24 h. Levels of these antibiotics were measured in the fluid of blisters resulting from application of cantharides. The concentration of ceftazidime in serum at 0.25 h after intravenous injection was 83....

Objective To illustrate a unique instance of Ehlers-Danlos syndrome type VIII (EDS VIII) with blood blister-like aneurysm of the middle cerebral artery. Design This is a single patient case report. Setting University of Wisconsin-Madison Hospital. Participants A 42-year-old woman with diagnosis of EDS VIII presented with a sudden onset severe headache and altered mental status. She was diagnose...

Blister formation and eccrine sweat gland necrosis is a cutaneous manifestation associated with states of impaired consciousness, most frequently reported after overdoses of central nervous system depressants, particularly phenobarbital. The case of a 45-year-old woman who developed "coma blisters" at six distinct anatomic sites after confirmed (laboratory) phenobarbital poisoning, associated w...