In this tutorial review we illustrate how the interaction of photoactive drugs/potential drugs with proteins or DNA in supramolecular complexes can determine the course of the reactions initiated by the drug absorbed photons, evidencing the mechanistic differences with respect to the solution conditions. We focus on photoprocesses, independent of oxygen, that lead to chemical modification of th...

Lanthanum (La) is considered a beneficial element, capable of inducing hormesis. Hormesis is a dose-response relationship phenomenon characterized by low-dose stimulation and high-dose inhibition. Herein we tested the effect of 0 and 10 μM La on growth and biomolecule concentrations of seedlings of four sweet bell pepper (Capsicum annuum L.) varieties, namely Sven, Sympathy, Yolo Wonder, and Zi...

Three-dimensional submicrometric structures and biomolecular patterns have been fabricated on a porous silicon film by an electron beam-based functionalization method. The immobilized proteins act as a passivation layer against material corrosion in aqueous solutions. The effects' dependence on the main parameters of the process (i.e., the electron beam dose, the biomolecule concentration, and ...

Cryo-electron microscopy（cryo-EM）is a powerful method to visualize high resolution structures of biomolecules under physiological condition. Since cryo-EM images represent conformational ensemble the target biomolecule, is applicable analyze biomolecular dynamics such as metastable conformations and/or free-energy landscape in changes. Here, we briefly introduce our deduce from and compare it w...

Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.

We study the transition to supramolecular ordering of a simple biostructural polysaccharide in water, and the structural properties of the ordered phase. Instabilities in aggregation and transport properties are observed across the transition. The possible role of the stereodynamics of the biomolecule-water system. as a whole is discussed in terms of the notion of connectivity pathways of hydro...

In this communication, we describe a novel synthetic method for fabricating multicomponent gold nanoparticles containing both gallium ions and biomolecules on the surface. Detailed compositional analysis, using STEM-HAADF and EELS spectroscopy, confirmed the crystalline nature of gold and chelation of gallium ions. The presence of the biomolecule was validated using conventional ELISA.

Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary rea...

By monitoring the Brownian motion of a fluorescent biomolecule of interest in free solution, information regarding its hydrodynamic volume as well as its solvent properties (i.e. viscosity) is obtained. While the translational diffusion of a fluorescent biomolecule, typically occurring on the microto millisecond time scale, is conveniently obtained from a conventional fluorescence correlation s...

Imaging techniques should differentiate between specific signals, from the biomolecules of interest, and non-specific signals, from the background. We present a probe containing (15)N and (14)N isotopes in approximately equal proportion, for secondary ion mass spectrometry imaging. This probe designed for a precise biomolecule analysis is insensitive to background signals.