AIM To determine frequency of tumors with immunohistochemical markers of cancer stem cells (CSC) CD44+/CD24- in patients with breast cancer (BC) of different molecular subtype and to evaluate their prognostic value. OBJECT Surgical material of 132 patients with BC stage I-II, age from 23 to 75 years, mean age - 50.2 ± 3.1 years was studied. METHODS Clinical, immunohistochemical (expression ...

Background & Aims: In recent years, molecular methods for characterizing genetic heterogeneity have found a major place in modern approaches. In this study, two different molecular techniques including Restriction Fragment Length Polymorphism (RFLP) and Multi Locus microsatellite typing (MLMT) were carried out in order to evaluate genetic heterogeneity among isolates of Leishmania major in Iran...

We study the accuracy of a Bayesian supervised method used to cluster individuals into genetically homogeneous groups on the basis of dominant or codominant molecular markers. We provide a formula relating an error criterion to the number of loci used and the number of clusters. This formula is exact and holds for arbitrary number of clusters and markers. Our work suggests that dominant markers...

The genetic architecture underlying the heritability of cardiovascular disease is incompletely understood. Metabolomics is an emerging technology platform that has shown early success in identifying biomarkers and mechanisms of common chronic diseases. Integration of metabolomics, genetics, and other omics platforms in a systems biology approach holds potential for elucidating novel genetic mar...

A cancer biomarker is a molecular signature that indicates the physiologic and pathologic changes in a particular tissue or cell type during cancer development. When such molecular signatures can be detected in the plasma (in this article, the term plasma is used to indicate plasma or serum), cancer biomarkers can have a significant effect on clinical outcomes. First, they can be used to detect...

Analyzing ambiguous bite marks using conventional morphological approaches to identify attackers is difficult; thus, applying molecular biological methods for identifying an attacker from their saliva a possible approach in forensic investigation. This study aimed establish oral bacterial DNA-based human and canine markers develop practical method discrimination. We considered Streptococcus ora...

Having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. In present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and RAPD markers. Significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...

An urgent problem in tomato breeding is the development of universal varieties and hybrids that combine high yields good fruit quality with group resistance to a number diseases. The purpose this study develop material resistant biotic stressors, basing on modern postgenomic technologies using molecular markers. At initial stage, we tested microsatellite markers from foreign literature sources,...

objective(s): iran is considered as one of the high-prevalence areas for β-thalassemia with a rate of about 10% carrier frequency. molecular diagnosis of the disease is performed both by direct sequencing and indirectly by the use of polymorphic markers present in the beta globin gene cluster. however, to date there is no reliable information on the application of the markers in the iranian pop...