One of the main challenges faced by biodetection systems is the ability to detect and identify a large range of toxins at low concentrations and in short times. Cell-based biosensors rely on detecting changes in cell behaviour, metabolism, or induction of cell death following exposure of live cells to toxic agents. Raman spectroscopy is a powerful technique for studying cellular biochemistry. D...

Interest in biosensors based on field-effect transistors (FETs), where an electrically operated gate controls the flow of charge through a semiconducting channel, is driven by the prospect of integrating biodetection capabilities into existing semiconductor technology. In a number of proposed FET biosensors, surface interactions with biomolecules in solution affect the operation of the gate or ...

A magnetoelastic (ME) platform coated with a biomolecular recognition element (bacteriophage) for selective and specific recognition of Bacillus anthracis spores is described. ME materials have a mass-sensitive, characteristic, resonance frequency. In response to the binding of spores to the phage on the ME biosensor, a corresponding decrease occurs in this resonance frequency, which enables th...

Noncoding RNAs (ncRNAs) have been found to have roles in a large variety of biological processes. Recent studies indicate that ncRNAs are far more abundant and important than initially imagined, holding great promise for use in diagnostic, prognostic, and therapeutic applications. Within ncRNAs, microRNAs (miRNAs) are the most widely studied and characterized. They have been implicated in initi...

M13 virus particles were covalently attached to a planar gold-coated quartz crystal microbalance (QCM) through reaction with a self-assembled monolayer of N-hydroxysuccinimide thioctic ester, followed by incorporation of the blocking agent bovine serum albumin. This immobilization chemistry produced a phage multilayer having a coverage equivalent to approximately 6.5 close-packed monolayers of ...

Steroidal estrogens are one of the most challenging classes of hazardous contaminants as they can cause adverse effects to biota in extremely low concentrations. They emerge in both waste waters and surface waters serving as a source of drinking water. Environmental Quality Standards for 17β-estradiol (E2) and 17α-ethinylestradiol (EE2), promulgated within the EU Water Framework Directive, are ...

BACKGROUND Fe₃O₄-gold-chitosan core-shell nanostructure can be used in biotechnological and biomedical applications such as magnetic bioseparation, water and wastewater treatment, biodetection and bioimaging, drug delivery, and cancer treatment. RESULTS Magnetite nanoparticles with an average size of 9.8 nm in diameter were synthesized using the chemical co-precipitation method. A gold-coated...

Modern breakthroughs in the fields of proteomics and DNA micro-arrays have widened the horizons of nanotechnology for applications with peptides and nucleic acids. Hence, biomimetic interest in the study and formulation of nanoscaled bio-structures, -materials, -devices and -therapeutic agent delivery vehicles has been recently increasing. Many of the currently–investigated functionalized bio-n...

The ability to manipulate the upconversion luminescence of lanthanide-ion doped fluoride upconversion nanoparticles (UCNPs) is particularly important and highly desired due to their wide applications in color displays, multiplexing bioassays and multicolor imaging. Here, we developed a strategy for simultaneously tuning color output and enhancing upconversion emission of Yb/Er doped fluoride UC...

This paper demonstrates a label-free biological sensing method usmg nanoporous polymer gratings. The high index modulation (0.07) of the nanoporous polymer grating structure generates a high signal-to-noise ratio, making the structure an ideal label-free biodetection platform. The fabrication process of the nanoporous polymeric grating involves holographic interference patterning and a function...