This study attempts to evaluate culture-dependent microbial and proximate composition of African salad (Abacha) sold at Nkpolu Oroworukwo (mile 3) market, Port Harcourt, Rivers State, Nigeria. Samples were bought from stationary vendors market within the months March, 2019 2021. Garnished (GAS) ungarnished (UAS) samples determined by standard microbiological techniques whereas (PC) was analysed...

In present pilot study aimed to estimate, presence of Mycobacterium avium subspecies paratuberculosis (MAP) antibodies in the human serum samples originating from North India using "Indigenous absorbed ELISA kit" (ELISA kit). The phase I, "ELISA kit" was optimized using protoplasmic antigen from native isolate of MAP "Indian Bison type" recovered from the biopsies of Crohn's disease patients. T...

OBJECTIVE. Healthcare-acquired infections (HAIs) cause substantial patient morbidity and mortality. Items in the environment harbor microorganisms that may contribute to HAIs. Reduction in surface bioburden may be an effective strategy to reduce HAIs. The inherent biocidal properties of copper surfaces offer a theoretical advantage to conventional cleaning, as the effect is continuous rather th...

Randomized controlled trials in wound care generalize poorly because they exclude patients with significant comorbid conditions. Research using real-world wound care patients is hindered by lack of validated methods to stratify patients according to severity of underlying illnesses. We developed a comprehensive stratification system for patients with wounds that predicts healing likelihood. Com...

Background: All wounds have unique and irregular topographies, including cavities where fluids and bacteria may collect and increase the risk of wound infection. Aims: To qualitatively and quantitatively investigate the relationship between the physical structure of antiseptic wound dressings and their ability to manage bioburden in models that simulate clinical conditions. Methods: In vitro mo...

Lung engineering is a potential alternative to transplantation for patients with end-stage pulmonary failure. Two challenges critical to the successful development of an engineered lung developed from a decellularized scaffold include (i) the suppression of resident infectious bioburden in the lung matrix, and (ii) the ability to sterilize decellularized tissues while preserving the essential b...

The differentiation between living and dead bacterial endospores is crucial in many research areas of microbiology. The identification of inactivated, non-pathogenic Bacillus anthracis spores is one reason why improvement of decontamination protocols is so desirable. Another field interested in spore viability is planetary protection, a sub-discipline of astrobiology that estimates the bioburde...

Hospital-acquired infections (HAIs) are responsible for clinical complications with an estimated 6.1% increase in attributable deaths [1,2]. HAIs increased the length of stay in a hospital from 5.9 to 9.6 days based on one study of over 1,250 patients [1]. Concomitantly, estimated healthcare costs double for patients with HAIs [1,3]. As many as 27% of hospital ward patient rooms are contaminate...

Normal wound healing in adults follows an ordered progression of events, which ultimately results in wound closure. In some cases, however, wound closure may not occur. There are many reasons why a wound will fail to heal, including both local (e.g. the presence of a foreign body or infection) and systemic (e.g. malnutrition or diabetes) factors. In a slow-healing or non-healing wound, it is im...