Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

In this study, the effect of temperature on the diffusion welding of AZ31 magnesium alloy and 7075 aluminum was discussed. Diffusion welding temperature was the most important parameter since all mechanisms are sensitive to temperature in Diffusion welding method. Intrusive contacts between the two alloys at temperatures of 430, and 440 °C 450 and took 60 min. In all tests constant pressure and...

SV40 T-Antigen binding site II was synthesized, cloned and analyzed for its ability to bind purified SV40 T-antigen. We report the binding constant of T-antigen for isolated site II. Using a filter binding assay the calculated binding constant was 6-8 fold less efficient than site I previously reported. Binding constants were calculated using two methods. The first was a direct calculation usin...

Equilibrium constants for the binding of ethyl (EIC), n-butyl (BIC), p-toluenesulphonylmethyl (TMIC) and 2,6-dimethylphenyl isocyanides (DIMPI) to an imidazole-haem complex in toluene and aqueous detergent micelle solutions were determined. In contrast to an earlier study, which indicated that the large affinities of myoglobin for binding DIMPI and 2,6-diethylphenylisocyanide (DEPI) relative to...

In the recent paper (2011) Faas and co-workers claimed that calmodulin could directly detect Ca 2+ signals by acting as extremely fast calcium buffer. However the results and their interpretation raise serious doubts about this conclusion. The major concerns are:-The on-rate constant for calcium binding by calmodulin (CaM) has not been directly measured and was instead found via Markovian fitti...