This study aimed to determine the effects of different concentrations and combinations of the phytohormones 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin, 6-benzylaminopurine (BAP), and 1-naphthaleneacetic acid (NAA) on callus induction and to demonstrate the role of elicitors and exogenous precursors on the production of mitragynine in a Mitragyna speciosa suspension culture. The best callu...

Mature embryos as explants are more convenient to operate and not subjected to time constraints in tissue culture systems. In this research, 120 sorghum varieties were screened to select an appropriate material for establishing a high efficient tissue culture system using mature embryos as explants. Loose, soft, and yellowish primary calluses were induced from the mature embryos of only one var...

Shoot tips of two fig cultivars (Conadria and Black Mission) proliferated in vitro, were cultured on modified MS medium supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The present investigation aimed to investigate the effect of different medium salt strengths (Double, full, half and quarter) and different sources of carbon (sucrose and fructose) at different concentrations (0.1 and 0.2 m...

In this study, we investigated the effect of plant growth regulators (PGRs) – auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me ...

Economically, rapeseed is one of the most important crops in the world. Over the past decades, rapeseed research has been focused on improving biotechnological methods to facilitate breeding. The effectiveness of these methods depends on efficient tissue culture techniques. The aim of the present study was to establish an efficient protocol for regeneration of rapeseed. It was conducted in thre...

An efficient protocol was developed for micro-propagation of Indian papaya (Carica papaya L.) var. Co7. Shoot bud induction occurred on culturing epicotyl segments in MS (Murashige and Skoog, 1962) medium, followed by transfer onto shoot multiplication, elongation and rooting media. Adventitious buds were induced on MS medium supplemented with varying concentration of thidiazuron (TDZ: 0.5-10 μ...

Nghiên cứu này được thực hiện để xây dựng quy trình tái sinh chồi cây dưa lưới in vitro từ các loại mô của mới nảy mầm. Kết quả nghiên cho thấy, thời gian thích hợp khử trùng hạt bằng dung dịch H2O2 15% là 9 phút với tỷ lệ mẫu sạch 80%, sống và mầm 100%. Mô lá, cuống lá đốt thân có khả năng cảm ứng phát hình thái cao hơn mầm, trụ dưới đỉnh. Môi trường tạo sẹo phiến môi cơ bản bổ sung 0,3 mg/L N...

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old nodal segment and leaf explants were cultured onto Murashige Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, 6-benzylaminopurine (BAP)) for shoot multiplication auxins (2,4-dichlorophenoxyacetic acid (2,4-D) picloram) callu...

Alzheimer’s disease (AD) is a multifactorial disease in which b-amyloid peptide (bAP) plays a critical role. We report here that the soluble fraction 1–40 of bAP differentially degrades protein kinase C-a and -g (PKCa and PKCg) isoenzymes in normal (age-matched controls, AC) and AD fibroblasts most likely through proteolytic cascades. Treatment with nanomolar concentrations of bAP(1–40) induced...